{"product_id":"ieej-efm16006","title":"Quantum dot linked immunosorbent assay using reduced antibody","description":"\u003cp\u003e\u003cstrong\u003eカテゴリ: \u003c\/strong\u003e研究会(論文単位)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e論文No: \u003c\/strong\u003eEFM16006\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eグループ名: \u003c\/strong\u003e【C】電子・情報・システム部門 電子材料研究会\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e発行日: \u003c\/strong\u003e2016\/01\/12\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eタイトル(英語): \u003c\/strong\u003eQuantum dot linked immunosorbent assay using reduced antibody \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e著者名: \u003c\/strong\u003eHikari Udaka(Saitama University),Takeshi Fukuda(Saitama University),Miho Suzuki(Saitama University)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e著者名(英語): \u003c\/strong\u003eUdaka Hikari(Saitama University),Fukuda Takeshi(Saitama University),Suzuki Miho(Saitama University)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eキーワード: \u003c\/strong\u003eQuantum dot|Antibody|quantum dot linked immunosorbent assay|Biological sensing device|Quantum dot|Antibody|quantum dot linked immunosorbent assay|Biological sensing device\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e要約(日本語): \u003c\/strong\u003eSince fluorescence-based biological imaging devices have been required for a long time, we investigated an optimized condition for synthesizing the CdSe\/ZnS quantum dot-partially partially reduced half antibody conj and its application to the quantum dot linked immunosorbent assay by using a conventional antigen-antibody reaction. Especially, we introduced two kinds of reducing agents, such as dithiothreitol and 2-mercaptoethylamine-HCl, for obtaining the half antibody immobilized on the CdSe\/ZnS quantum dot. Once establishing the reaction condition, the detection limit of IL-6 antigen concentration was 0.5 ng\/mL, and this value is low enough for practical applications.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e要約(英語): \u003c\/strong\u003eSince fluorescence-based biological imaging devices have been required for a long time, we investigated an optimized condition for synthesizing the CdSe\/ZnS quantum dot-partially partially reduced half antibody conj and its application to the quantum dot linked immunosorbent assay by using a conventional antigen-antibody reaction. Especially, we introduced two kinds of reducing agents, such as dithiothreitol and 2-mercaptoethylamine-HCl, for obtaining the half antibody immobilized on the CdSe\/ZnS quantum dot. Once establishing the reaction condition, the detection limit of IL-6 antigen concentration was 0.5 ng\/mL, and this value is low enough for practical applications.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e原稿種別: \u003c\/strong\u003e英語\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePDFファイルサイズ: \u003c\/strong\u003e742 Kバイト\u003c\/p\u003e","brand":"IEEJ-PDF","offers":[{"title":"PDFダウンロード（一般価格330円\/会員価格220円） \/ A4 \/ 4","offer_id":46362911703279,"sku":"IEEJ-EFM16006-PDF","price":330.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/9512\/2159\/files\/IEEJ-PDF_913dbabe-3352-49d8-8110-0467af881852.png?v=1743644297","url":"https:\/\/ieej.bookpark.ne.jp\/products\/ieej-efm16006","provider":"電気学会 電子図書館","version":"1.0","type":"link"}