DIUTHAME offers higher reproducibility than DHB, a widely used matrix in FT-ICR mass spectrometry imaging analysis

カテゴリ: 研究会(論文単位)

論文No: DEI22040,EFM22005

グループ名: 【A】基礎・材料・共通部門 誘電・絶縁材料/【C】電子・情報・システム部門 電子材料合同研究会

発行日: 2022/02/07

タイトル(英語): DIUTHAME offers higher reproducibility than DHB, a widely used matrix in FT-ICR mass spectrometry imaging analysis

著者名: 瀬藤 光利(浜松医科大学)

著者名(英語): Mitsutoshi Setou(Hamamatsu University School of Medicine)

キーワード: DIUTHAME-MS|FT-ICR|MALDI-MS|PC|MSI| mass accuracy

要約(日本語): DIUTHAME, a matrix-free ionization-assisting substrate, was tested for mass accuracy and intensity using standard lipid and mouse brain sections. DIUTHAME and DHB were applied to individual brain sections on ITO slides. Subsequently, PC(18:2/18:2) was poured over the DIUTHAME and matrix. Finally, samples were measured with FTICR-MS. DIUTHAME-MS showed significantly lower mass errors than MALDI-MS on both standard and endogenous PCs. DIUTHAME-MS revealed higher intensity for all endogenous PC ions. DIUTHAME outperforms MALDI-MS in mass accuracy and intensity reproducibility in standard lipid and mouse brain tissue analyses. DIUTHAME can be preferable to MS and MSI studies for mass accuracy and intensity.

要約(英語): DIUTHAME, a matrix-free ionization-assisting substrate, was tested for mass accuracy and intensity using standard lipid and mouse brain sections. DIUTHAME and DHB were applied to individual brain sections on ITO slides. Subsequently, PC(18:2/18:2) was poured over the DIUTHAME and matrix. Finally, samples were measured with FTICR-MS. DIUTHAME-MS showed significantly lower mass errors than MALDI-MS on both standard and endogenous PCs. DIUTHAME-MS revealed higher intensity for all endogenous PC ions. DIUTHAME outperforms MALDI-MS in mass accuracy and intensity reproducibility in standard lipid and mouse brain tissue analyses. DIUTHAME can be preferable to MS and MSI studies for mass accuracy and intensity.

本誌: 2022年2月10日誘電・絶縁材料/電子材料合同研究会

本誌掲載ページ: 11-13 p

原稿種別: 英語

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