Photoinjection of DNA plasmids into intact Tobacco BY-2 cells

カテゴリ: 研究会(論文単位)

論文No: OQD22008

グループ名: 【C】電子・情報・システム部門 光・量子デバイス研究会

発行日: 2022/03/25

タイトル(英語): Photoinjection of DNA plasmids into intact Tobacco BY-2 cells

著者名: Priyo Jatmiko Muhammad Ridho(Nara Institute of Science and Technology),Ueda Yuma(Nara Institute of Science and Technology),Rukmana Taufiq Indra(Nara Institute of Science and Technology),Tadashi Kunieda(Nara Institute of Science and Technology),Demura Taku

著者名(英語): Muhammad Ridho Priyo Jatmiko(Nara Institute of Science and Technology),Yuma Ueda(Nara Institute of Science and Technology),Taufiq Indra Rukmana(Nara Institute of Science and Technology),Kunieda Tadashi(Nara Institute of Science and Technology),Taku Demura

キーワード: photoinjection|Tobacco BY-2 cells|femtosecond laser|fluorescence labeled DNA plasmid|photoinjection|Tobacco BY-2 cells|femtosecond laser|fluorescence labeled DNA plasmid

要約(日本語): This work demonstrates the photoinjection of DNA plasmids into Tobacco BY-2 cells by a small pore formation using single femtosecond laser pulse. The injection process was visualized by utilizing fluorescence-labeled DNA plasmid. The injection was investigated with osmotic pressure control and pH control of culture medium. The osmotic pressure control was utilized to reduce the turgor pressure. Decreasing pH of culture medium reduces the interaction between DNA plasmid and the cell wall and softens the cell wall. By optimizing these effects due to the osmotic pressure and pH conditions, DNA plasmids could be injected into intact Tobacco BY-2 cell.

要約(英語): This work demonstrates the photoinjection of DNA plasmids into Tobacco BY-2 cells by a small pore formation using single femtosecond laser pulse. The injection process was visualized by utilizing fluorescence-labeled DNA plasmid. The injection was investigated with osmotic pressure control and pH control of culture medium. The osmotic pressure control was utilized to reduce the turgor pressure. Decreasing pH of culture medium reduces the interaction between DNA plasmid and the cell wall and softens the cell wall. By optimizing these effects due to the osmotic pressure and pH conditions, DNA plasmids could be injected into intact Tobacco BY-2 cell.

本誌: 2022年3月28日光・量子デバイス研究会

本誌掲載ページ: 5-8 p

原稿種別: 英語

PDFファイルサイズ: 415 Kバイト