Mixing Two Solutions in PDMS SlipChip for Dynamic Study of Protein Reactions

カテゴリ: 研究会(論文単位)

論文No: BMS24015

グループ名: 【E】センサ・マイクロマシン部門 バイオ・マイクロシステム研究会

発行日: 2024/07/01

タイトル(英語): Mixing Two Solutions in PDMS SlipChip for Dynamic Study of Protein Reactions

著者名: IMRAN MD Hussain Mohammad(Toyohashi University of Technology),INAAM Rafia(Toyohashi University of Technology),NAKASONE Yusuke(Kyoto University),OKAMOTO Shunya (Toyohashi University of Technology),SHIBATA Takayuki (Toyohashi University of Technology),NAGAI

著者名(英語): MD Hussain Mohammad IMRAN(Toyohashi University of Technology),Rafia INAAM(Toyohashi University of Technology),Yusuke NAKASONE(Kyoto University),Shunya OKAMOTO(Toyohashi University of Technology),Takayuki SHIBATA(Toyohashi University of Technology),Moeto NAGAI(Toyohashi University of Technology)

キーワード: SlipChip|Microfluidics|Protein Dynamics|Diffusion|SlipChip|Microfluidics|Protein Dynamics|Diffusion

要約(日本語): A PDMS SlipChip enables the precise mixing of two solutions on a microliter scale to study dynamic protein reactions. It facilitates solution blending, prevents leakage, and adjusts solution volume to maintain microchannel integrity. This was achieved through a PDMS solution mixer holding frame with eight circular legs, enhancing soft lithography accuracy and enabling the production of flat PDMS SlipChip. We showcased a high-precision control device for top and bottom alignment, using a micro-gauge to enhance x-axis and y-axis movements, improving free interface diffusion accuracy. We adjusted the soft-lithography baking time to modify PDMS surfaces, eliminating stiction for smooth sliding, preventing leakage, and enabling easy sample handling with high-throughput analysis.

要約(英語): A PDMS SlipChip enables the precise mixing of two solutions on a microliter scale to study dynamic protein reactions. It facilitates solution blending, prevents leakage, and adjusts solution volume to maintain microchannel integrity. This was achieved through a PDMS solution mixer holding frame with eight circular legs, enhancing soft lithography accuracy and enabling the production of flat PDMS SlipChip. We showcased a high-precision control device for top and bottom alignment, using a micro-gauge to enhance x-axis and y-axis movements, improving free interface diffusion accuracy. We adjusted the soft-lithography baking time to modify PDMS surfaces, eliminating stiction for smooth sliding, preventing leakage, and enabling easy sample handling with high-throughput analysis.

本誌: 2024年7月4日-2024年7月5日バイオ・マイクロシステム研究会

本誌掲載ページ: 23-27 p

原稿種別: 英語

PDFファイルサイズ: 1,466 Kバイト