Optoporation of Cells Encapsulated in Collagen Gel with Color Resist Micropatterns

カテゴリ: 研究会(論文単位)

論文No: BMS24017

グループ名: 【E】センサ・マイクロマシン部門 バイオ・マイクロシステム研究会

発行日: 2024/07/01

タイトル(英語): Optoporation of Cells Encapsulated in Collagen Gel with Color Resist Micropatterns

著者名: Khan Mehdi Ali(Toyohashi University of Technology),Mishra Aniket(Toyohashi University of Technology),OKAMOTO Shunya (Toyohashi University of Technology),SHIBATA Takayuki (Toyohashi University of Technology),NAGAI Moeto (Toyohashi University of Technology

著者名(英語): Mehdi Ali Khan (Toyohashi University of Technology),Aniket Mishra(Toyohashi University of Technology),Shunya OKAMOTO(Toyohashi University of Technology),Takayuki SHIBATA(Toyohashi University of Technology),Moeto NAGAI(Toyohashi University of Technology)

キーワード: Intracellular Delivery|Optoporation|Collagen Matrix|PR-254|Suspension cells|Intracellular Delivery|Optoporation|Collagen Matrix|PR-254|Suspension cells

要約(日本語): Optoporation using color resist micropatterns represents a significant advancement in the field of targeted cellular therapies. Improving the effective delivery of biomolecules into suspended cells is a key focus in biomedical research, particularly for applications such as cancer immunotherapy; however, it faces the difficulty of intracellular delivery. Here, we demonstrate a novel method for intracellular delivery into suspension cells using collagen gel and color resist micropatterns. The approach involves embedding cells in a collagen gel matrix, which is then seeded onto a color resist micropatterns device. These micropatterns allow controlled membrane disruption by the formation of laser-triggered bubbles, creating temporary membrane perforations for efficient delivery of extracellular cargo. Its compatibility with 3D cell cultures holds promise for applications in tissue engineering and personalized medicine.

要約(英語): Optoporation using color resist micropatterns represents a significant advancement in the field of targeted cellular therapies. Improving the effective delivery of biomolecules into suspended cells is a key focus in biomedical research, particularly for applications such as cancer immunotherapy; however, it faces the difficulty of intracellular delivery. Here, we demonstrate a novel method for intracellular delivery into suspension cells using collagen gel and color resist micropatterns. The approach involves embedding cells in a collagen gel matrix, which is then seeded onto a color resist micropatterns device. These micropatterns allow controlled membrane disruption by the formation of laser-triggered bubbles, creating temporary membrane perforations for efficient delivery of extracellular cargo. Its compatibility with 3D cell cultures holds promise for applications in tissue engineering and personalized medicine.

本誌: 2024年7月4日-2024年7月5日バイオ・マイクロシステム研究会

本誌掲載ページ: 35-39 p

原稿種別: 英語

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