蛍光分子封入リポソームアレイセンサを用いたターゲットタンパク質の検出

カテゴリ: 部門大会

論文No: 29pm3-PS-109

グループ名: 第32回「センサ・マイクロマシンとその応用システム」シンポジウム論文集

発行日: 2015/10/21

タイトル(英語): Discrimination of Target Proteins using Array Sensor with Liposome Encapsulating Fluorescent Molecules

著者名: 今村 亮太*{1}，張* 子洋{1}，島内 寿徳{2}，村田 直樹{1}，山下 馨{1}，福澤 理行{1}，野田 実{1}({1}京都工芸繊維大学，{2}岡山大学)

キーワード: biosensor,fluorescence,liposome,biomolecule,protein

要約(英語): This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizaing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different loposomes independent from each other. In this work we discriminated the species and concentration of target proteins by the method: Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their different concentrations were clearly discriminated. In addition, the extent of discrimination was improved by denaturation of the protein, as the denatured protein strongly interacts with loposome membranes because of the change in conformation states of protein.

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