Structural Characterization of DNA origami with different crossover arrangement

カテゴリ: 研究会(論文単位)

論文No: BMS16023

グループ名: 【E】センサ・マイクロマシン部門 バイオ・マイクロシステム研究会

発行日: 2016/06/29

タイトル(英語): Structural Characterization of DNA origami with different crossover arrangement

著者名: Ma Zhipeng(Kyoto University),Huang Yunfei(Kyoto University),Park Seongsu(Kyoto University),Yamashita Naoki(Kyoto University),Hirai Yoshikazu(Kyoto University),Tsuchiya Toshiyuki(Kyoto University),Yamada Hirofumi(Kyoto University),Tabata Osamu(Osaka University),Kawai Kentaro(Osaka University),Kim Do-Nyun(Seoul National University)

著者名(英語): Zhipeng Ma(Kyoto University),Yunfei Huang(Kyoto University),Seongsu Park(Kyoto University),Naoki Yamashita(Kyoto University),Yoshikazu Hirai(Kyoto University),Toshiyuki Tsuchiya(Kyoto University),Hirofumi Yamada(Kyoto University),Osamu Tabata(Kyoto University),Kentaro Kawai(Osaka University),Do-Nyun Kim(Seoul National University)

キーワード: DNA origami|nanotechnology|crossover|frequency-modulation atomic force microscopy

要約(日本語): DNA origami is an increasingly attractive method to form nanostructures due to its potential for a wide range of applications that utilize them as nanomechanical components for the creation of micro/nano systems. However, the effective use highly depends on the understanding of their structural and mechanical properties, especially the knowledge of their dependence on the crossovers and nicks which are basic units for DNA origami. In this paper, the density-varied crossover regions of DNA origami were quantitatively characterized using a super-resolution frequency-modulation atomic force microscopy (FM-AFM). The observation indicated that the crossover structure is more flexible than the dsDNA helix, such that most of the deformation caused by electrostatic repulsion occurs at the crossover positions.

要約(英語): DNA origami is an increasingly attractive method to form nanostructures due to its potential for a wide range of applications that utilize them as nanomechanical components for the creation of micro/nano systems. However, the effective use highly depends on the understanding of their structural and mechanical properties, especially the knowledge of their dependence on the crossovers and nicks which are basic units for DNA origami. In this paper, the density-varied crossover regions of DNA origami were quantitatively characterized using a super-resolution frequency-modulation atomic force microscopy (FM-AFM). The observation indicated that the crossover structure is more flexible than the dsDNA helix, such that most of the deformation caused by electrostatic repulsion occurs at the crossover positions.

原稿種別: 英語

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