Development of 3D Parallel Single-Cell Printer Based on Deterministic Encapsulation

カテゴリ: 研究会(論文単位)

論文No: BMS18021

グループ名: 【E】センサ・マイクロマシン部門 バイオ・マイクロシステム研究会

発行日: 2018/07/13

タイトル(英語): Development of 3D Parallel Single-Cell Printer Based on Deterministic Encapsulation

著者名: BHARDWAJ ROHIT(Toyohashi University of Technology),TANAGI KENTARO(Toyohashi University of Technology),PANDEY GAURAV(Toyohashi University of Technology),SHIBATA TAKAYUKI(Toyohashi University of Technology),NAGAI MOETO(Toyohashi University of Technology)

著者名(英語): ROHIT BHARDWAJ(Toyohashi University of Technology),KENTARO TANAGI(Toyohashi University of Technology),GAURAV PANDEY(Toyohashi University of Technology),TAKAYUKI SHIBATA(Toyohashi University of Technology),MOETO NAGAI(Toyohashi University of Technology)

キーワード: Single cell manipulation|Sequential Trapping|Cell encapsulation|Droplet generation|Multi-layer Photolithography|Fabrication of hanging Structure|Single cell manipulation|Sequential Trapping|Cell encapsulation|Droplet generation|Multi-layer Photolithography|Fabrication of hanging Structure

要約(日本語): When a cell is encapsulated in a droplet, the droplet acts like a container. Growth and behaviour of the cell depend upon the environment provided by the droplet. This study focuses on 3D parallel single cell printer for arranging cells in 3D structure to overcome the heterogeneity of cell population. Developed 3D micronozzle array shows sequential filling and ejection of droplets. To achieve the same generated droplet was stored and flowed into micronozzle array. We have been working for the development of encapsulation of single cells. Moreover, we introduce the development of a device that allows high efficiency of trapping, encapsulation, and retrieval. This device provides the base which will help in the analysis of tissues at the single cell leve.

要約(英語): When a cell is encapsulated in a droplet, the droplet acts like a container. Growth and behaviour of the cell depend upon the environment provided by the droplet. This study focuses on 3D parallel single cell printer for arranging cells in 3D structure to overcome the heterogeneity of cell population. Developed 3D micronozzle array shows sequential filling and ejection of droplets. To achieve the same generated droplet was stored and flowed into micronozzle array. We have been working for the development of encapsulation of single cells. Moreover, we introduce the development of a device that allows high efficiency of trapping, encapsulation, and retrieval. This device provides the base which will help in the analysis of tissues at the single cell leve.

原稿種別: 英語

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