Spatiotemporal dynamics of evoked neuronal responses induced by a focused femtosecond laser

カテゴリ: 部門大会

論文No: SS2-8

グループ名: 【C】2022年電気学会電子・情報・システム部門大会

発行日: 2022/08/24

タイトル(英語): Spatiotemporal dynamics of evoked neuronal responses induced by a focused femtosecond laser

著者名: 瀬川 夕海（大阪公立大学・大阪市立大学）,箕嶋 渉（大阪公立大学・大阪市立大学）,増井 恭子（大阪公立大学・大阪市立大学）,細川 千絵（大阪公立大学・大阪市立大学）

著者名(英語): Yumi Segawa (Osaka Metropolitan University / Osaka City University),Wataru Minoshima (Osaka Metropolitan University / Osaka City University),Kyoko Masui (Osaka Metropolitan University / Osaka City University),Chie Hosokawa (Osaka Metropolitan University / Osaka City University)

キーワード: フェムト秒レーザー|培養神経回路網|カルシウムイメージング|細胞外電位多点計測|シナプス結合|femtosecond laser|cultured neuronal networks|calcium imaging|multi-site extracellular recording|synaptic connections

要約(日本語): Neurons form networks via synaptic connections. Spatiotemporal dynamics of neuronal activities are modified with external stimuli, related to memory and learning. As a novel stimulation method, we demonstrated noninvasive, single-neuron stimulation with a focused femtosecond laser. Laser-evoked responses were analyzed with fluorescence Ca²⁺ imaging and multi-site extracellular recording. 
A femtosecond laser (center wavelength 800 nm, pulse width ~100 fs, repetition rate 82 MHz) was focused to cultured neurons, and then intracellular Ca²⁺ drastically increased. Further, network-wide electrical responses were induced for 60 ms. Our results suggest that a femtosecond laser irradiation is effective to reveal the spatiotemporal dynamics of neuronal activities.