Flow-induced Ca2+ signaling in proximal tubule cells extracted from kidney organoids on a microphysiological system

カテゴリ: 部門大会

論文No: 15P2-P-48

グループ名: 【E】第39回「センサ・マイクロマシンと応用システム」シンポジウム

発行日: 2022/11/07

タイトル(英語): Flow-induced Ca2+ signaling in proximal tubule cells extracted from kidney organoids on a microphysiological system

著者名: MA CHENG(京都大学), Banan Sadeghian Ramin(京都大学), 藤本 和也(京都大学), 川上 瑛彦(京都大学), 荒岡 利和(京都大学), 横川 隆司(京都大学)

著者名(英語): CHENG MA(Kyoto University), Ramin Banan Sadeghian(Kyoto University), Kazuya Fujimoto(Kyoto University), Akihiko Kawakami(Kyoto University), Toshikazu Araoka(Kyoto University), Ryuji Yokokawa(Kyoto University),

キーワード: hiPSC|ADPKD|micro physiological systems|proximal tubule|flow-induced Ca2+ signaling|hiPSC|ADPKD|micro physiological systems|proximal tubule|flow-induced Ca2+ signaling

要約(日本語): We report a kidney proximal tubule-on-a-chip by seeding hiPSC-derived cells for the first time showing higher expression levels of proximal tubule proteins over those of immortalized cells (RPTECs). Alongside, we demonst

要約(英語): We report a kidney proximal tubule-on-a-chip by seeding hiPSC-derived cells for the first time showing higher expression levels of proximal tubule proteins over those of immortalized cells (RPTECs). Alongside, we demonstrated the change of flow-induced Ca2+ signaling under different flow-induced shear stress values. This can be helpful in our ongoing project of on-chip autosomal dominant polycystic kidney disease (ADPKD) modeling by comparing with patient-derived iPS cells.

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