Odor Biosensor Using Odorant Binding Protein and Olfactory Receptor

カテゴリ: 研究会(論文単位)

論文No: CHS19027

グループ名: 【E】センサ・マイクロマシン部門 ケミカルセンサ研究会

発行日: 2019/07/02

タイトル(英語): Odor Biosensor Using Odorant Binding Protein and Olfactory Receptor

著者名: Deng Hongchao(Tokyo Institute of Technology),Sukekawa Yuji(Tokyo Institute of Technology),Mitsuno Hidefumi(University of Tokyo),Kanzaki Ryohei(University of Tokyo),Cali Khasim(University of Manchester),Persaud Krishna(University of Manchester),Nakamoto Takamichi(Tokyo Institute of Technology)

著者名(英語): Hongchao Deng(Tokyo Institute of Technology),Yuji Sukekawa(Tokyo Institute of Technology),Hidefumi Mitsuno(University of Tokyo),Ryohei Kanzaki(University of Tokyo),Khasim Cali(University of Manchester),Krishna Persaud(University of Manchester),Takamichi Nakamoto(Tokyo Institute of Technology)

キーワード: Biosensor|Odor|Olfactory receptor|Odorant binding protein

要約(日本語): An odor biosensor has already been established based on insect olfactory receptor (OR) and fluorescent protein in our previous work. Under the stimulation of corresponding ligands, the fluorescent intensity change shows a concentration-dependence feature. In this system, however, the detection threshold still has big gap with insect, also the hydrophobic ligand is dissolved in dimethyl sulphoxide (DMSO) instead of carried by odorant binding protein (OBP). Thus, we intend to apply the mosquito OBP-WTAgamOBP1 to imitate the biological olfactory system as well as improve the sensitivity. From the result, we can acquire some insight into the selectivity of OBP to various odorants.

要約(英語): An odor biosensor has already been established based on insect olfactory receptor (OR) and fluorescent protein in our previous work. Under the stimulation of corresponding ligands, the fluorescent intensity change shows a concentration-dependence feature. In this system, however, the detection threshold still has big gap with insect, also the hydrophobic ligand is dissolved in dimethyl sulphoxide (DMSO) instead of carried by odorant binding protein (OBP). Thus, we intend to apply the mosquito OBP-WTAgamOBP1 to imitate the biological olfactory system as well as improve the sensitivity. From the result, we can acquire some insight into the selectivity of OBP to various odorants.

原稿種別: 英語

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