多細胞からのIn vivo自動細胞内記録

カテゴリ: 部門大会

論文No: TC1-23

グループ名: 【C】平成25年電気学会電子・情報・システム部門大会講演論文集

発行日: 2013/09/04

タイトル(英語): Automated intracellular recording from multiple neurons in vivo

著者名: 太田桂輔 (矢崎),松元 崇(矢崎),杉山陽子 (沖縄科学技術大学院大学),鈴木 崇之(理化学研究所),鴨志田 敦史(理化学研究所,日本ナショナルインスツルメンツ),日本ナショナルインスツルメンツ (理化学研究所),村山 正宜(理化学研究所)

著者名(英語): Keisuke Ota(RIKEN),Takashi Matsumoto(RIKEN),Yoko Yazaki(OIST),Takayuki Suzuki(RIKEN),Atsushi Kamoshida(RIKEN,National Instruments Japan Corporation),National Instruments Japan Corporation (RIKEN),Masanori Murayama(RIKEN)

キーワード: 細胞内記録|微小電極法|多細胞記録|In vivo記録|閾値下膜電位|Intracellular recording|Microelectrode method|Multicellular recording|In vivo recording|subthreshold membrane potential

要約(日本語): Multiple in vivo intracellular recording is a useful technique for understanding how neurons and neural circuits function. However, this is formidable tasks for researchers. To overcome this difficulty, we developed an automated intracellular recording (AIR) system. This AIR system can automatically move an electrode in the brain, find a neuron and record its intracellular potential. We evaluated the performance of the AIR system in anesthetized head-restrained mice. The success rate for one electrode was 63 % (n=11 electrodes). The average stable recording time was 56 min, and a maximum time was 193 min. Additionally, we run 6 AIR systems in parallel and succeeded in simultaneous recording from 4 neurons. This system will help us to ascertain signal transmission between neurons in vivo.

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