抗体固定化基板を用いた蛍光異方性測定光学系の開発

カテゴリ: 部門大会

論文No: TC10-6

グループ名: 【C】平成26年電気学会電子・情報・システム部門大会講演論文集

発行日: 2014/09/03

タイトル(英語): Construction of Optical System for Fluorescence Anisotropy Measurement Used for Immobilized Antibody Substrate

著者名: 鈴木 誠一(成蹊大学),長谷川 世納(成蹊大学),伊藤 拓哉(成蹊大学),菅沼 秀樹(成蹊大学),加藤 茂(成蹊大学),小島 紀徳(成蹊大学)

著者名(英語): Seiichi Suzuki(Seikei University),Sena Hasegawa(Seikei University),Takuya Ito(Seikei University),Hideki Suganuma(Seikei University),Shigeru Kato(Seikei University),Toshinori Kojima(Seikei University)

キーワード: 免疫センサ|蛍光異方性|エバネッセント励起|固定化抗体|Immunosensor|Flurescence anisotropy|Evanescent excitation|Immobilized antibodysu

要約(日本語): Immunosensor has high sensitivity and specificity of antibody. In this study antigen-antibody binding is detected by fluorescence anisotropy change of fluorescently labeled antibody. With binding of antigen, molecular motion of antibody Fab region is restricted and fluorescence anisotropy increases. This sensing scheme is theoretically free from the background noise of surface adsorption. A evanescent excitation optical stage was constructed for antibody immobilized substrate measurement. Feasibility of this method was investigated by the measurements of fluorescence anisotropy of sensor substrate by evanescent excitation system. Anisotropy of immobilized antibody was changed by administration of antigen molecule. Sensitivity of the system was lower than 1 pg/mL.

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