Quantum dot linked immunosorbent assay using reduced antibody

カテゴリ: 研究会(論文単位)

論文No: DEI16018

グループ名: 【A】基礎・材料・共通部門 誘電・絶縁材料研究会

発行日: 2016/01/12

タイトル(英語): Quantum dot linked immunosorbent assay using reduced antibody

著者名: Hikari Udaka(Saitama University),Takeshi Fukuda(Saitama University),Miho Suzuki(Saitama University)

著者名(英語): Udaka Hikari(Saitama University),Fukuda Takeshi(Saitama University),Suzuki Miho(Saitama University)

キーワード: Quantum dot|Antibody|quantum dot linked immunosorbent assay|Biological sensing device|Quantum dot|Antibody|quantum dot linked immunosorbent assay|Biological sensing device

要約(日本語): Since fluorescence-based biological imaging devices have been required for a long time, we investigated an optimized condition for synthesizing the CdSe/ZnS quantum dot-partially partially reduced half antibody conj and its application to the quantum dot linked immunosorbent assay by using a conventional antigen-antibody reaction. Especially, we introduced two kinds of reducing agents, such as dithiothreitol and 2-mercaptoethylamine-HCl, for obtaining the half antibody immobilized on the CdSe/ZnS quantum dot. Once establishing the reaction condition, the detection limit of IL-6 antigen concentration was 0.5 ng/mL, and this value is low enough for practical applications.

要約(英語): Since fluorescence-based biological imaging devices have been required for a long time, we investigated an optimized condition for synthesizing the CdSe/ZnS quantum dot-partially partially reduced half antibody conj and its application to the quantum dot linked immunosorbent assay by using a conventional antigen-antibody reaction. Especially, we introduced two kinds of reducing agents, such as dithiothreitol and 2-mercaptoethylamine-HCl, for obtaining the half antibody immobilized on the CdSe/ZnS quantum dot. Once establishing the reaction condition, the detection limit of IL-6 antigen concentration was 0.5 ng/mL, and this value is low enough for practical applications.

原稿種別: 英語

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