Increasing the sensitivity of enzyme-linked immunosorbent assay for latex allergens using gold nanoparticles

カテゴリ: 研究会(論文単位)

論文No: DEI20012,EFM20012

グループ名: 【A】基礎・材料・共通部門 誘電・絶縁材料/【C】電子・情報・システム部門 電子材料合同研究会

発行日: 2020/01/22

タイトル(英語): Increasing the sensitivity of enzyme-linked immunosorbent assay for latex allergens using gold nanoparticles

著者名: Phengdaam Apichat(Prince of Songkla University),Traisakul Thunkamol (Prince of Songkla University),Phupet Benjaporn (Prince of Songkla University),Runsaeng Phanthipha (Prince of Songkla University),Ekgasit Sanong(Chulalongkorn University)

著者名(英語): Apichat Phengdaam(Prince of Songkla University),Thunkamol Traisakul(Prince of Songkla University),Benjaporn Phupet(Prince of Songkla University),Phanthipha Runsaeng(Prince of Songkla University),Sanong Ekgasit(Chulalongkorn University)

キーワード: ELISA|gold nanoparticles|latex allergy|ELISA|gold nanoparticles|latex allergy

要約(日本語): The latex allergy is lethal case in allergy patients due to immune system in human against the latex allergens from natural rubber. Therefore, determination of latex allergens in natural rubber products is necessary. For determination of protein in latex, enzyme-linked immunosorbent assay (ELISA) was employed. The reaction of tetramethylbenzidine and peroxidase enzyme on ELISA were used as colorimetric sensor. In this work, gold nanoparticles (AuNPs), that have strong light scattering and catalytic properties, will be used as substrate for enhancing sensitivity of ELISA for latex allergens. The relationship of AuNP morphology and sensitivity of ELISA will be investigated.

要約(英語): The latex allergy is lethal case in allergy patients due to immune system in human against the latex allergens from natural rubber. Therefore, determination of latex allergens in natural rubber products is necessary. For determination of protein in latex, enzyme-linked immunosorbent assay (ELISA) was employed. The reaction of tetramethylbenzidine and peroxidase enzyme on ELISA were used as colorimetric sensor. In this work, gold nanoparticles (AuNPs), that have strong light scattering and catalytic properties, will be used as substrate for enhancing sensitivity of ELISA for latex allergens. The relationship of AuNP morphology and sensitivity of ELISA will be investigated.

原稿種別: 英語

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