培養心筋組織に対する慢性的電気刺激の効果

カテゴリ: 研究会(論文単位)

論文No: MBE11116

グループ名: 【C】電子・情報・システム部門 医用・生体工学研究会

発行日: 2011/09/20

タイトル(英語): Effects of chronic electrical stimulation on cultured ventricular myocytes

著者名: 森 雅英(東京大学),武内 彬正(東京大学),白 冰(東京大学),北川 加奈(東京大学),榛葉 健太(東京大学),高山 祐三(東京大学),小谷 潔(東京大学),神保 泰彦(東京大学)

著者名(英語): Mori Masahide(University of Tokyo),Takeuchi Akimasa(University of Tokyo),Bai Bing(University of Tokyo),Kitagawa Kana(University of Tokyo),Shimba Kenta(University of Tokyo),Takayama Yuzo(University of Tokyo),Kotani Kiyoshi(University of Tokyo),Jimbo Yasuhiko(University of Tokyo)

要約(日本語): Precisely controlled spatio-temporal electrical stimulation is one of the promising methods for low-invasive arrhythmia treatment. In this work, we studied effects of chronic stimulation on spontaneous contraction rhythms of cultured cardiomyocytes. Cardiomyocytes were taken from new-born rats and cultured on microelectrode-array (MEA) substrates. Electrical stimulation, the interval of which was a little shorter than the spontaneous contraction, was applied through the embedded electrodes of the MEAs and the spontaneous rhythms were compared between before and after stimulation. Externally applied rhythms were successfully induced by chronic stimulation for three hours. The results suggested that the contraction rhythm could be controlled by externally applied chronic electrical stimulation. And to investigate any changes of cardiomyocytes induced by electrical stimulation in detail, we tried to investigate differences in gene expressions of ion channel proteins in cardiac myocytes using reverse transcription polymerase chain reaction(RT-PCR).

原稿種別: 日本語

PDFファイルサイズ: 679 Kバイト