神経細胞の分散培養系にイオンチャネル一体型嗅覚受容体を発現させた匂いバイオセンサーの提案と実現性の検証

カテゴリ: 研究会(論文単位)

論文No: MBE11118

グループ名: 【C】電子・情報・システム部門 医用・生体工学研究会

発行日: 2011/09/20

タイトル(英語): Feasibility study of odor biosensor using dissociate neuronal culture with gene expression of ionotropic odorant receptors

著者名: 棚田 法男(東京大学),櫻井 健志(東京大学),光野 秀文(東京大学),ダグラス バッカム(東京大学),神埼 亮平(東京大学),高橋 宏知(東京大学)

著者名(英語): Tanada Norio(The Univ. of Tokyo),Sakurai Takeshi(The Univ. of Tokyo),Mitsuno Hidefumi(The Univ. of Tokyo),Douglous Bakkum(The Univ. of Tokyo),Kanzaki Ryohei(The Univ. of Tokyo),Takahashi Hirokazu(The Univ. of Tokyo)

要約(日本語): We had previously proposed a novel odor biosensor by expressing ionotropic odorant receptors of insects into dissociated cultures of neurons of rats. The odorant-gated ion channel structure of insect odorant receptor could realize easy functional expression into cells. The neuronal dissociated cultures of rats have three significant advantages: a long lifetime comparable to rats, i.e., a few years; amplification ability from weak ionic currents of odorant receptors into easily detectable action potentials of neurons. In the present work, we investigated whether dissociated cultures with transected cells could change the spatio-temporal activities in response to different odor stimuli. We demonstrate that cultures with 10% of pheromone-receptor-expressed cells showed synchronous increase of calcium signals at the presentation of pheromone in both transfected cells and non-transfected cells and that the activity pattern was dose-dependent. Thus, these results demonstrated that, in our proposed senseor, discrimination of odorants from spatiotemporal activity pattern is possible.

原稿種別: 日本語

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