汎用型大規模細胞改変システムのための装置及び細胞膜穿孔体の開発

カテゴリ: 研究会(論文単位)

論文No: MBE11139

グループ名: 【C】電子・情報・システム部門 医用・生体工学研究会

発行日: 2011/09/21

タイトル(英語): Compact High Performance Cell Membrane Perforator System

著者名: 柏﨑 脩平(秋田県立大学),山本 隆寛(秋田県立大学),齋藤 敬(秋田県立大学)

著者名(英語): Kashiwazaki Syuhei(Akita Prefectural University),Yamamoto Takahiro(Akita Prefectural University),Saito Takashi(Akita Prefectural University)

要約(日本語): Recently, the importance of artificial cell modification technology becomes drastically.Our high performance cell membrane perforation technology which perforates the cell membrane byphotochemical reaction has a two benefit. First, the technology employs extremely simple pinpointoxidation process. Secondly, the perforation system is similar to printing instruments so it is easily adapted for automation and scale-up. Therefore, we will expand the technology for e.g. regenerative medicine and cell therapy. In this study, we developed a compact perforation system and cell membrane perforator. The system is designed as simple enough for labtool with spring pressurization, Chebyshev-linkage, and servo-motor. The perforator is made by nano imprintedPolydimethylsiloxane (PDMS) casted on the mold. The former perforator was fragile by air bubbleswhich were unavoidable by the process but we solved the problem and improved the manufactureefficiency. As a result, we could successfully apply the system to PC12 rat pheochromocytoma cells and introduced hydrophilic fluorescence dye Alexa594 into them. Our technology is established to expand multipurpose of the cell membrane perforation.

原稿種別: 日本語

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