Investigation of the Annealing Process Parameters for Discrete and Polymerized DNA Origami Structures

カテゴリ: 研究会(論文単位)

論文No: MSS15009

グループ名: 【E】センサ・マイクロマシン部門 マイクロマシン・センサシステム研究会

発行日: 2015/07/03

タイトル(英語): Investigation of the Annealing Process Parameters for Discrete and Polymerized DNA Origami Structures

著者名: 馬 志鵬(京都大学),朴 晟洙(京都大学),山下 直輝(京都大学),平井 義和(京都大学),土屋 智由(京都大学),田畑 修(京都大学)

著者名(英語): Zhipeng Ma(Kyoto University),Seongsu Park(Kyoto University),Naoki Yamashita(Kyoto University),Yoshikazu Hirai(Kyoto University),Toshiyuki Tsuchiya(Kyoto University),Osamu Tabata(Kyoto University)

キーワード: ＤＮＡ　ｏｒｉｇａｍｉ|ｎａｎｏｓｔｒｕｃｔｕｒｅ|ｎａｎｏｔｅｃｈｎｏｌｏｇｙ|ｓｅｌｆ－ａｓｓｅｍｂｌｙ|ａｔｏｍｉｃ　ｆｏｒｃｅ　ｍｉｃｒｏｓｃｏｐｙ|DNA origami|nanostructure|nanotechnology|self-assembly|atomic force microscopy

要約(日本語): A unique approach for the polymerization of DNA origami through the double crossovers of staple strands is proposed for achieving rigid higher-order DNA architectures, eventually utilized as a component for MEMS and NEMS. Based on this, a new DNA origami with bivalve configuration that can form discrete and polymerized structures simultaneously owing to its conformational flexibility is constructed. The self-assembly behavior of bivalve origami is investigated with varying the annealing process parameters. Our findings focus on determining the annealing process parameters that facilitate the formation of discrete and polymerized origami, respectively. The results provide the insight into the underlying mechanism of DNA origami self-assembly, revealing its dependence on scaffold/staple design strategy and the annealing process parameters.

要約(英語): A unique approach for the polymerization of DNA origami through the double crossovers of staple strands is proposed for achieving rigid higher-order DNA architectures, eventually utilized as a component for MEMS and NEMS. Based on this, a new DNA origami with bivalve configuration that can form discrete and polymerized structures simultaneously owing to its conformational flexibility is constructed. The self-assembly behavior of bivalve origami is investigated with varying the annealing process parameters. Our findings focus on determining the annealing process parameters that facilitate the formation of discrete and polymerized origami, respectively. The results provide the insight into the underlying mechanism of DNA origami self-assembly, revealing its dependence on scaffold/staple design strategy and the annealing process parameters.

原稿種別: 英語

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