Hybrid subtractive - additive femtosecond laser processing for ship-in-a-bottle integration of 3D polymer patterns inside glass micro-channels for single cell monitoring

カテゴリ: 研究会(論文単位)

論文No: OQD15014

グループ名: 【C】電子・情報・システム部門 光・量子デバイス研究会

発行日: 2015/03/06

タイトル(英語): Hybrid subtractive - additive femtosecond laser processing for ship-in-a-bottle integration of 3D polymer patterns inside glass micro-channels for single cell monitoring

著者名: シーマ フェリクス(理化学研究所),呉 東(理化学研究所),徐 剣(理化学研究所),緑川 克美(理化学研究所), 杉岡 幸次(理化学研究所)

著者名(英語): Felix Sima(RIKEN),Dong Wu(RIKEN),Jian Xu(RIKEN),Katsumi Midorikawa(RIKEN),Koji Sugioka(RIKEN)

キーワード: femtosecond laser processing|two photon polymerization|Foturan glass|negative-tone photoresist|ship-in-a-bottle polymer integration|lab-on-a-chip device

要約(日本語): Microfluidic devices are nowadays attractive tools for cell biology research because they offer accurate control of cellular environment and are promising surroundings for analyzing sub-cellular content at the single-cell level. Polymeric micro and nanostructure pattern integration inside microfluidic systems offers concrete functionalities such as nano-mechanical manipulation on both 2D and 3D environments, increase of sensitivity, optical visualization and eventually increase the performance of lab-on-a-chip devices. We propose herein the “ship-in-a-bottle” concept based on a hybrid subtractive and additive femtosecond laser processing in order to integrate three dimensional polymer structures inside 3D glass micro-channels. It consists of Femtosecond Laser Assisted Wet Etching (FLAE) of a photosensitive glass followed by Two-Photon Polymerization (TPP) of a negative epoxy-resin which allows lowering the size limit to smaller details, below cell dimension. Both glass channels and polymeric integrated patterns are 3D spatially designed for cell monitoring and analysis. The spatial control of resin solidification by photo‐polymerization permits the design of sub-micrometric channels used for single cell trapping, live monitoring and analysis. The adjustable channel sizes and diffusible chemo-attractant gradients could help in understanding single cell-type specific mechanical as well as signaling aspects during migration.

要約(英語): Microfluidic devices are nowadays attractive tools for cell biology research because they offer accurate control of cellular environment and are promising surroundings for analyzing sub-cellular content at the single-cell level. Polymeric micro and nanostructure pattern integration inside microfluidic systems offers concrete functionalities such as nano-mechanical manipulation on both 2D and 3D environments, increase of sensitivity, optical visualization and eventually increase the performance of lab-on-a-chip devices. We propose herein the “ship-in-a-bottle” concept based on a hybrid subtractive and additive femtosecond laser processing in order to integrate three dimensional polymer structures inside 3D glass micro-channels. It consists of Femtosecond Laser Assisted Wet Etching (FLAE) of a photosensitive glass followed by Two-Photon Polymerization (TPP) of a negative epoxy-resin which allows lowering the size limit to smaller details, below cell dimension. Both glass channels and polymeric integrated patterns are 3D spatially designed for cell monitoring and analysis. The spatial control of resin solidification by photo‐polymerization permits the design of sub-micrometric channels used for single cell trapping, live monitoring and analysis. The adjustable channel sizes and diffusible chemo-attractant gradients could help in understanding single cell-type specific mechanical as well as signaling aspects during migration.

原稿種別: 英語

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