Saturated excitation microscopy using image subtraction

カテゴリ: 研究会(論文単位)

論文No: OQD17005

グループ名: 【C】電子・情報・システム部門 光・量子デバイス研究会

発行日: 2017/03/06

タイトル(英語): Saturated excitation microscopy using image subtraction

著者名: Nawa Yasunori(Osaka University),Yonemaru Yasuo(Osaka University),Kasai Atsushi(Osaka University),Smith Nicholas(Osaka University),Hashimoto Hitoshi(Osaka University),Kawata Satoshi(Osaka University),Fujita Katsumasa(Osaka University)

著者名(英語): Yasunori Nawa(Osaka University),Yasuo Yonemaru(Osaka University),Atsushi Kasai(Osaka University),Nicholas Smith(Osaka University),Hitoshi Hashimoto(Osaka University),Satoshi Kawata(Osaka University),Katsumasa Fujita(Osaka University)

キーワード: Nonlinear optics|Fluorescence|Super-resolution|Nonlinear optics|Fluorescence|Super-resolution

要約(日本語): We report the improvement of signal to noise ratio in saturated excitation microscope using image subtraction method. In this method, specimens are scanned 2 or 3 times with different excitation intensities that induce different level of saturation in fluorescence excitation. Fluorescence signal nonlinearly responding to the excitation intensity is extracted subtracting the fluorescence images. Compared with our previous method, the 8- or 32-fold increase of detectable signal were theoretically estimated for extracting the 2nd - or 3rd- order nonlinear fluorescence components. Super-resolution imaging of stained HeLa cells and mouse brain tissue was demonstrated by using the proposed method.

要約(英語): We report the improvement of signal to noise ratio in saturated excitation microscope using image subtraction method. In this method, specimens are scanned 2 or 3 times with different excitation intensities that induce different level of saturation in fluorescence excitation. Fluorescence signal nonlinearly responding to the excitation intensity is extracted subtracting the fluorescence images. Compared with our previous method, the 8- or 32-fold increase of detectable signal were theoretically estimated for extracting the 2nd - or 3rd- order nonlinear fluorescence components. Super-resolution imaging of stained HeLa cells and mouse brain tissue was demonstrated by using the proposed method.

原稿種別: 英語

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