Development of Laser Ionization Techniques Using Zeolite Matrix for Imaging Multiple Drugs Administered in Cancer Cells

カテゴリ: 研究会(論文単位)

論文No: OQD17014

グループ名: 【C】電子・情報・システム部門 光・量子デバイス研究会

発行日: 2017/03/06

タイトル(英語): Development of Laser Ionization Techniques Using Zeolite Matrix for Imaging Multiple Drugs Administered in Cancer Cells

著者名: Nomura Shusei(Osaka university),Hazama Hisanao(Osaka university),Kaneda Yasufumi(Osaka university),Fujino Tatsuya(Toyo university),Awazu Kunio(Osaka university)

著者名(英語): Shusei Nomura(Osaka university),Hisanao Hazama(Osaka university),Yasufumi Kaneda(Osaka university),Tatsuya Fujino(Toyo university),Kunio Awazu(Osaka university)

キーワード: imaging mass spectrometry|matrix-assisted laser desorption/ionization|zeolite matrix|anticancer drug|photosensitizer|cancer cells|imaging mass spectrometry|matrix-assisted laser desorption/ionization|zeolite matrix|anticancer drug|photosensitizer|cancer cells

要約(日本語): In order to evaluate the efficacy of combined therapy using chemotherapy and photodynamic therapy, it is necessary to assess the uptakes of an anticancer drug and a photosensitizer in cancer cells. We employed imaging mass spectrometry using matrix-assisted laser desorption/ionization to simultaneously observe the distributions of multiple drugs. When zeolite matrix made by mixing a conventional matrix, 6-aza-2-thiothymine, and zeolite NaY5.6 was used, the ionization efficiency of the anticancer drug, docetaxel, was improved about 13-fold, and the simultaneous detection of docetaxel and the photosensitizer, protoporphyrin IX, administered in human prostate cancer cells was succeeded by dropping the zeolite matrix solution onto the dried spot of the cell suspension.

要約(英語): In order to evaluate the efficacy of combined therapy using chemotherapy and photodynamic therapy, it is necessary to assess the uptakes of an anticancer drug and a photosensitizer in cancer cells. We employed imaging mass spectrometry using matrix-assisted laser desorption/ionization to simultaneously observe the distributions of multiple drugs. When zeolite matrix made by mixing a conventional matrix, 6-aza-2-thiothymine, and zeolite NaY5.6 was used, the ionization efficiency of the anticancer drug, docetaxel, was improved about 13-fold, and the simultaneous detection of docetaxel and the photosensitizer, protoporphyrin IX, administered in human prostate cancer cells was succeeded by dropping the zeolite matrix solution onto the dried spot of the cell suspension.

原稿種別: 英語

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