Effect of Intense Pulsed Ultraviolet Irradiation on the Proliferation of HeLa Cells

カテゴリ: 研究会(論文単位)

論文No: PPT11077

グループ名: 【A】基礎・材料・共通部門 パルスパワー研究会

発行日: 2011/12/15

タイトル(英語): Effect of Intense Pulsed Ultraviolet Irradiation on the Proliferation of HeLa Cells

著者名: LU Peng(熊本大学),渡辺 哲也(熊本大学),光武 和典(熊本大学),木下 由美(熊本大学),勝木 淳(熊本大学),秋山 秀典(熊本大学)

著者名(英語): LU Peng(Kumamoto University),Watanabe Tetsuya(Kumamoto University),Mitsutake Kazunori(Kumamoto University),Kishita Yumi(Kumamoto University),Katsuki Sunao(Kumamoto University),Akiyama Hidenori(Kumamoto University)

キーワード: Ｐｕｌｓｅｄ　ＵＶ|ＨｅＬａ　ｃｅｌｌ|ｐｒｏｌｉｆｅｒａｔｉｏｎ|ｖｉａｂｉｌｉｔｙ|Ｃａｓｐａｓｅ－３／７　ａｃｔｉｖａｔｉｏｎ|Pulsed UV|HeLa cell|proliferation|viability|Caspase-3/7 activation

要約(日本語): In this paper, we present the response of HeLa cells exposed to the intense pulsed ultraviolet radiation (PUV). PUV source is driven by a pulse current (120 ns) short capillary discharge. The measured spectrum ranges from the vacuum UV to UVA. After the exposure, we examine the irradiation effect on the cell viability and proliferation. Dead cells were detected by the fluorescent dye propidium iodide (PI). A real time cell imaging system was employed to monitor the cell growth. Also, Caspase-3/7 activation of HeLa cells caused by PUV was measured temporally. Experimental results show that cell survival rate decreased rapidly when the number of pulse exceeded about 300 shots. 800 pulses cause about 50% dead cells. HeLa cell proliferation shows a hormetic mode response to the pulse number. Cell growth was inhibited by large number of pulses. While, it is notable that cell proliferation was promoted after exposure to 10 and 50 pulses PUV induced proliferation change of HeLa cells was also discussed preliminary in the term of Caspase-3/7 activation caused by irradiation.

要約(英語): In this paper, we present the response of HeLa cells exposed to the intense pulsed ultraviolet radiation (PUV). PUV source is driven by a pulse current (120 ns) short capillary discharge. The measured spectrum ranges from the vacuum UV to UVA. After the exposure, we examine the irradiation effect on the cell viability and proliferation. Dead cells were detected by the fluorescent dye propidium iodide (PI). A real time cell imaging system was employed to monitor the cell growth. Also, Caspase-3/7 activation of HeLa cells caused by PUV was measured temporally. Experimental results show that cell survival rate decreased rapidly when the number of pulse exceeded about 300 shots. 800 pulses cause about 50% dead cells. HeLa cell proliferation shows a hormetic mode response to the pulse number. Cell growth was inhibited by large number of pulses. While, it is notable that cell proliferation was promoted after exposure to 10 and 50 pulses PUV induced proliferation change of HeLa cells was also discussed preliminary in the term of Caspase-3/7 activation caused by irradiation.

原稿種別: 日本語

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