非対称バーストパルスシステムによるメダカ受精卵への物質導入実験における周囲溶液の影響調査

カテゴリ: 論文誌(論文単位)

グループ名: 【A】基礎・材料・共通部門

発行日: 2015/09/01

タイトル(英語): Effects of Surrounding Solutions on Material Incorporation into Fertilized Medaka Eggs by using Asymmetric Burst Pulse System

著者名: 河野　晋（有明工業高等専門学校 電気工学科）,米田　宗次（有明工業高等専門学校 電気工学科）,山口　明美（有明工業高等専門学校 物質工学科）,福原　義剛（有明工業高等専門学校 電気工学科）,冨永　伸明（有明工業高等専門学校 物質工学科）

著者名(英語): Susumu Kono (Department of Electrical Engineering, National Institute of Technology, Ariake College), Munetsugu Yoneda (Department of Electrical Engineering, National Institute of Technology, Ariake College), Akemi Yamaguchi (Department of Chemical Science and Engineering, National Institute of Technology, Ariake College), Yoshitake Fukuhara (Department of Electrical Engineering, National Institute of Technology, Ariake College), Nobuaki Tominaga (Department of Chemical Science and Engineering, National Institute of Technology, Ariake College)

キーワード: メダカ,受精卵,物質導入,卵膜,シクロヘキシミド,ナノ秒パルス,バーストパルス　　medaka,fertilized egg,material incorporation,chorion,cycloheximide,nanosecond pulse,burst pulse

要約(英語): To establish a simple method for incorporating materials into fertilized medaka eggs by applying electric pulses including nanosecond pulsed electric fields (nsPEFs), we have tested pulse parameters as pulse width, pulse intensity, number of pulses, combination of several pulses, interval of pulses, and polarities of pulses. Improvement in incorporation efficiency was achieved with a new pulse system, named asymmetric burst system, which could generate a short rectangular pulse (from ten to several hundreds nanosecond) with high intensity (several kilovolt) and asymmetric bipolar burst pulses (in a range of microsecond to millisecond) with low intensity (from several volt to several tens volt). To gain the total efficiency for incorporation, it is necessary to investigate other factors in addition to the pulse parameters. In this paper, the effects of solutions that surrounds eggs are investigated. Among the combinations of pulsing and immersed surrounding solutions, we found that the electrical and experimental deleterious effects were strongly reduced under the condition that eggs were pulsed in a 6 mS/cm NaCl solution and kept in the same solution or put into and kept in an isotonic solution for 2 hours. This might be due to an appropriate electrical conductivity and an osmotic pressure difference between the inside of an egg and the surrounding solution. Moreover the adverse effects of cycloheximide on embryogenesis appeared clearly using the same condition.

本誌: 電気学会論文誌A（基礎・材料・共通部門誌） Vol.135 No.9 （2015）

本誌掲載ページ: 542-547 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejfms/135/9/135_542/_article/-char/ja/