ナノ秒パルス電界の周波数解析に基づくがん細胞のアポトーシス誘導と電気刺激量の関係

カテゴリ: 論文誌(論文単位)

グループ名: 【A】基礎・材料・共通部門

発行日: 2024/11/01

タイトル(英語): Relationship between Apoptosis Induction in Cancer Cells and the Amount of Electrical Stimulation on Frequency Analysis of Nanosecond Pulsed Electric Field

著者名: 蜷川　悠斗（東京都立大学），杉浦　廉（東京都立大学），加藤　英子（東京都立大学），八木　一平（東京都立大学），永田　晃基（東京都立産業技術研究センター），山岡　英彦（東京都立産業技術研究センター），立花　孝介（大分大学），小田　昭紀（千葉工業大学），内田　諭（東京都立大学）

著者名(英語): Yuto Ninagawa (Tokyo Metropolitan University), Ren Sugiura (Tokyo Metropolitan University), Eiko Kato (Tokyo Metropolitan University), Ippei Yagi (Tokyo Metropolitan University), Kohki Nagata (Tokyo Metropolitan Industrial Technology Research Institute), Hidehiko Yamaoka (Tokyo Metropolitan Industrial Technology Research Institute), Kosuke Tachibana (Oita University), Akinori Oda (Chiba Institute of Technology), Satoshi Uchida (Tokyo Metropolitan University)

キーワード: ナノ秒パルス電界，アポトーシス，周波数解析，がん細胞，消費エネルギー，パルス幅　　nanosecond pulsed electric field，apoptosis，frequency analysis，cancer cell，energy consumption，pulse width

要約(英語): Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a non-invasive cancer treatment with minimal side effects. However, the relationship between the electrical stress induced in cells by the external electric field and the biological response has not been fully investigated quantitatively. We investigated the effects of nsPEF therapy on cancer cells using microgap electrodes under low voltage conditions. We developed an equivalent circuit model using intracellular components to determine the amount of stimulation of cellular components and performed frequency analysis of nsPEF. nsPEF applied to Jurkat cells activated caspase-3 and induced apoptosis at shorter pulse widths. nsPEF induced biological responses and apoptosis in cancer cells. The relationship between the biological response induced by nsPEF and the amount of electrical stimulation was investigated by comparing the experimental results with frequency analysis. Using quantitative values for each cellular element, the relationship between the biological response and the amount of electrical stimulation can be integrated.

本誌: 電気学会論文誌A（基礎・材料・共通部門誌） Vol.144 No.11 （2024）

本誌掲載ページ: 407-414 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejfms/144/11/144_407/_article/-char/ja/