Glucose Dependency of the Metabolic Pathway of HEK 293 Cells Measured by a Flow-through Type pH/CO2 Sensor System Using ISFETs

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2011/09/01

タイトル(英語): Glucose Dependency of the Metabolic Pathway of HEK 293 Cells Measured by a Flow-through Type pH/CO2 Sensor System Using ISFETs

著者名: Akira Yamada (Department of Mechanical Systems Engineering, Faculty of Engineering, Hiroshima Institute of Technology/Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University), Satoshi Mohri (Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University/Physiology 1, Kawasaki Medical School), Michihiro Nakamura (Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University), Keiji Naruse (Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University)

著者名(英語): Akira Yamada (Department of Mechanical Systems Engineering, Faculty of Engineering, Hiroshima Institute of Technology/Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University), Satoshi Mohri (Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University/Physiology 1, Kawasaki Medical School), Michihiro Nakamura (Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University), Keiji Naruse (Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University)

キーワード: ion-sensitive field effect transistor (ISFET)，cellular activity，metabolism，glycolysis，respiration，HEK 293

要約(英語): Our group previously reported the application of a flow-through type pH/CO2 sensor system designed to evaluate the metabolic activity of cultured cells. The sensor system consists of two ion-sensitive field effect transistors (ISFETs), an ISFET to measure the total pH change and an ISFET enclosed within a gas-permeable silicone tube to measure the pH change attributable to CO2. In that study, we used the system to quantitatively analyze metabolic switching induced by glucose concentration changes in three cultured cell types (bovine arterial endothelium cell (BAEC), human umbilical vein endothelium cell (HUVEC), and rat cardiomuscle cell (RCMC)), and to measure the production rates of total carbonate and free lactic acid in the cultured cells. In every cell type examined, a decrease in the glucose concentration led to an increase in total carbonate, a product of cellular respiration, and a decrease of free lactic acid, a product of glycolysis. There were very significant differences among the cell types, however, in the glucose concentrations at the metabolic switching points. We postulated that the cell has a unique switching point on the metabolic pathway from glycolysis to respiration. In this paper we use our sensor system to evaluate the metabolic switching of human embryonic kidney 293 cells triggered by glucose concentration changes. The superior metabolic pathway switched from glycolysis to respiration when the glucose concentration decreased to about 2 mM. This result was very similar to that obtained in our earlier experiments on HUVECs, but far different from our results on the other two cells types, BAECs and RCMCs. This sensor system will be useful for analyzing cellular metabolism for many applications and will yield novel information on different cell types.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.131 No.9 （2011） 特集：医療・ヘルスケアにおける工学技術の新展開

本誌掲載ページ: 1535-1539 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/131/9/131_9_1535/_article/-char/ja/