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神経細胞移植in vitro評価のための共培養デバイス試作

神経細胞移植in vitro評価のための共培養デバイス試作

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カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2011/11/01

タイトル(英語): Co-culture Devices for in vitro Monitoring of Neural Transplantation Processes

著者名: 伊藤 宏樹(東京大学大学院 新領域創成科学研究科),榛葉 健太(東京大学大学院 新領域創成科学研究科),武内 彬正(東京大学大学院 新領域創成科学研究科),高山 祐三(東京大学大学院 情報理工学研究科),小谷 潔(東京大学大学院 新領域創成科学研究科),神保 泰彦(東京大学大学院 新領域創成科学研究科)

著者名(英語): Hiroki Ito (Graduate School of Frontier Sciences, The University of Tokyo), Kenta Shimba (Graduate School of Frontier Sciences, The University of Tokyo), Akimasa Takeuchi (Graduate School of Frontier Sciences, The University of Tokyo), Yuzo Takayama (Graduate School of Information Science and Technology, The University of Tokyo), Kiyoshi Kotani (Graduate School of Frontier Sciences, The University of Tokyo), Yasuhiko Jimbo (Graduate School of Frontier Sciences, The University of Tokyo)

キーワード: 微小電極アレイ基板,神経回路,パターン化培養,マイクロ構造物,再生医療  microelectrode array,neuronal network,patterned culture,microstructure,tissue engineering

要約(英語): Transplantation of stem cell-derived neurons into the damaged neuronal tissue is one of the promising methods for the recovery of neuronal functions. However, little is known about the signal transmission between transplanted stem cell-derived neuronal networks and the host brain tissue during recovery. In this study, we developed microelectrode-array (MEA) substrates with multiple micro-chambers for cell cultures. Each micro-chamber consisted of 4 cell-plating spots with interconnecting conduits fabricated by polydimethylsiloxane (PDMS) lithography. The conduits had the tunneling structures, the height of which was approximately 6 μm, through which only neurites could grow into adjacent cell-plating spots. We cultured mouse cortical neurons in the subset of cell-plating spots, and stem cell-derived neurons were injected into the residual spots 7 days after starting incubation of primary cultures. Intracellular calcium transients as well as electrical activity were detected from both primary cultured-cells. And extracellular electrical activities were recorded from both primary cultured-cells and stem cell-derived neurons in the co-cultures. These results suggested that our MEA-based co-culture system would be a useful tool for in vitro monitoring of transplantation processes of stem cell-derived neurons into host neuronal tissue.

本誌: 電気学会論文誌C(電子・情報・システム部門誌) Vol.131 No.11 (2011) 特集:電気関係学会関西連合大会

本誌掲載ページ: 1983-1989 p

原稿種別: 論文/日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/131/11/131_11_1983/_article/-char/ja/

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