軟磁性材料を用いた培養神経回路網への局所誘導電流刺激

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2012/04/01

タイトル(英語): Localized Induced Current Stimulation to Neuronal Culture Using Soft Magnetic Material

著者名: 斎藤　淳史（東京大学　大学院新領域創成科学研究科），斎藤　亜希（東京大学　大学院新領域創成科学研究科），森口　裕之（東京大学　大学院新領域創成科学研究科），小谷　潔（東京大学　大学院新領域創成科学研究科），神保　泰彦（東京大学　大学院新領域創成科学研究科）

著者名(英語): Atsushi Saito (Graduate School of Frontier Sciences, University of Tokyo), Aki Saito (Graduate School of Frontier Sciences, University of Tokyo), Hiroyuki Moriguchi (Graduate School of Frontier Sciences, University of Tokyo), Kiyoshi Kotani (Graduate School of Frontier Sciences, University of Tokyo), Yasuhiko Jimbo (Graduate School of Frontier Sciences, University of Tokyo)

キーワード: 培養神経回路網，細胞パターニング，微小電極アレイ，同期バースト現象，誘導電流刺激　　cultured neuronal networks，cell patterning，microelectrode arrays，periodic synchronized bursting，induced current stimulation

要約(英語): To establish precisely focused magnetic stimulation, we developed a Mu-meal based low-frequency localized induced current (LIC) stimulation system with micro-fabricated dual cell-culture chamber. The dual cell-culture chamber was arranged in a concentric circle manner. Between the inner and outer chambers, 4 or 8 connecting micro-channels were fabricated using polydimethylsiloxane (PDMS). Rat cortical neurons were separately cultured in outer and inner chambers. Through the micro-channels, functional synaptic connections were formed. Mu-metal that has very high magnetic permeability was aligned along the outer circle, which allowed us of LIC stimulation to the cells in the outer chamber. Applying low-frequency magnetic fields to the Mu-metal, induced currents were generated and the electrical activity of the cells in the outer chamber was modified depending on the stimulation intensity. Following the modified activity in the outer circles, the cells in the inner chamber also showed slightly depressed activity patterns. These results suggested that our system would be promising for localized stimulation of neuronal networks and highly regulation of network activities.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.132 No.4 （2012）

本誌掲載ページ: 509-515 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/132/4/132_4_509/_article/-char/ja/