流路形状による電界の絞り込みを用いたセルソータの特性改善

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2012/10/01

タイトル(英語): Improvement of DEP Cell Sorter by the Design of Flow Channel for Higher Field Gradient

著者名: 鈴木　誠一（成蹊大学理工学部物質生命理工学科），伊藤　恵美子（成蹊大学理工学部物質生命理工学科），長谷川　世納（成蹊大学理工学部物質生命理工学科），高橋　勉（成蹊大学理工学部物質生命理工学科），原　孝彦（東京都医学総合研究所）

著者名(英語): Seiichi Suzuki (Faculty of Sci. & Tech., Seikei University), Emiko Ito (Faculty of Sci. & Tech., Seikei University), Sena Hasegawa (Faculty of Sci. & Tech., Seikei University), Tsutomu Takahashi (Faculty of Sci. & Tech., Seikei University), Takahiko Hara (Tokyo Metropolitan Institute of Medical Science)

キーワード: セルソータ，誘電泳動，微小流路，電界勾配　　Cell Sorter，Dielectrophoresis，Micro-Channel，Field Gradient

要約(英語): In this study, a high separation ratio micro-fluidic cell sorting by dielectrophoretic (DEP) force is presented. Resent development of iPS cell technology has promoted the possibility of regenerative therapy. In technologies of genetic cell processing requires purification of specific target cells by cell sorter. Most prevailing system for cell separation is the flow cytometry (FCM), which has been developed for research purpose. In spite of the high performance in separation ratio, it takes rather high cost in one operation. For clinical use, a device contacting biological specimen must be disposable to prevent cross infection. A low cost micro-fluidic cell sorter, using DEP force, can be fabricated by photolithography technique. However, sorting rate of existing DEP cell sorter is far lower than that of FCM because of the limitation of applicable voltage in aqueous fluid. To circumvent the problem and raise the DEP force, implementation of field gradient dependence was investigated. The design of flow channel was deiced to make high gradient at the separation point, and enhance the DEP force. Distribution of the electric field was proved to have a peak intensity at the entrance of separation channel by numerical calculated of Finite Element Method. DEP separation of 2μm diameter latex beads at the rate of 3.0mm/sec, 6 times large than that of our previous system, was demonstrated.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.132 No.10 （2012） 特集：医療関連電子技術のニュートピック

本誌掲載ページ: 1595-1601 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/132/10/132_1595/_article/-char/ja/