セロトニンによる培養神経回路網の発火パターン調節

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2013/09/01

タイトル(英語): Serotonergic Modulation of Activity Pattern on Neuronal Network

著者名: 榛葉　健太（東京大学大学院 新領域創成科学研究科），有松　和之（東京大学大学院 新領域創成科学研究科），磯村　拓哉（東京大学大学院 新領域創成科学研究科），武内　彬正（東京大学大学院 新領域創成科学研究科），小谷　潔（東京大学大学院 新領域創成科学研究科），神保　泰彦（東京大学大学院 新領域創成科学研究科）

著者名(英語): Kenta Shimba (Graduate School of Frontier Schiences, The University of Tokyo), Kazuyuki Arimatsu (Graduate School of Frontier Schiences, The University of Tokyo), Takuya Isomura (Graduate School of Frontier Schiences, The University of Tokyo), Akimasa Takeuchi (Graduate School of Frontier Schiences, The University of Tokyo), Kiyoshi Kotani (Graduate School of Frontier Schiences, The University of Tokyo), Yasuhiko Jimbo (Graduate School of Frontier Schiences, The University of Tokyo)

キーワード: セロトニン，培養神経回路網，微小電極アレイ，同期バースト，移動エントロピー　　serotonin，neuronal network，microelectrode array，synchronized bursting，transfer entropy

要約(英語): Serotonin (5-hydroxy tryptamine; 5-HT) plays major roles in mood and cognition. Dysfunction of 5-HT system leads to serious mental illnesses such as major depressive disorder. Little is known, however, about how 5-HT modulates the activity of neuronal network. Here, we evaluated the effects of 5-HT on the activity of cultured neurons obtained from the prefrontal cortex. Dissociated neurons were cultured on a microelectrode array (MEA), and stimulated using 5-HT and selective 5-HT receptor (5-HTR) agonists (8-OH-DPAT and 2-Me-5-HT were used as 5-HTR1A and 5-HTR3 agonist, respectively). The bursting activity generated from the cultured neurons was recorded from the electrodes of the MEA. The inter burst intervals (IBI) increased after 5-HT and agonists treatment. Moreover, we evaluated functional connectivity using a delayed transfer entropy method. Pharmacological treatment resulted in enhancement of the connectivity in the entire network. Agonists had apparent effects as the changes of IBI and functional connectivity compared with 5-HT. These results suggest that 5-HT has roles in enhancement of IBI and functional connectivity via 5-HTR1A and 5-HTR3.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.133 No.9 （2013） 特集：エージェントシミュレーションの最新動向

本誌掲載ページ: 1814-1819 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/133/9/133_1814/_article/-char/ja/