培養神経回路網における自発性神経電気活動のグルコース濃度依存性

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2013/10/01

タイトル(英語): The Glucose Concentration-dependency of Spontaneous Activity in a Cultured Neuronal Network

著者名: 箕嶋　渉（関西学院大学 理工学部 人間システム工学科），伊東　嗣功（関西学院大学 理工学部 人間システム工学科），工藤　卓（関西学院大学 理工学部 人間システム工学科）

著者名(英語): Wataru Minoshima (Department of Human System Interaction, School of Science and Technology, Kwansei Gakuin University), Hidekatsu Ito (Department of Human System Interaction, School of Science and Technology, Kwansei Gakuin University), Suguru Kudoh (Department of Human System Interaction, School of Science and Technology, Kwansei Gakuin University)

キーワード: 培養神経回路網，分散培養，グルコース代謝，多点計測システム，自発性神経活動　　Cultured Neuronal Network，Dissociated Culture，Glucose Metabolism，Multi Electrode Array (MEA)，Spontaneous Activity

要約(英語): Cultured rat hippocampal network on a multi electrode array (MEA) dish is a useful model for analyzing network electrical dynamics and its developmental changes. Neurons autonomously form a complex network on a MEA dish and spontaneous electrical activity is often observed without any input from the external world. The spontaneous activity is generated by synaptic interactions between neurons and reflects an internal biochemical state of a whole neuronal network. The origin of electrical activity is electrochemical potential generated by active transport of ions, which requires catabolism of ATP. We elucidated the relationship between spontaneous electrical activity and external glucose concentration. The spontaneous activity changed transiently, depending on glucose concentrations. The number of electrical spikes in spontaneous activity increases depending on the concentration of external glucose concentration. Interestingly, this increase is not only suppressed but turn to decrease. In the case of glucose concentration is 17.56 mM, the number of activity is the most, and then it decreases in the case of glucose concentration is more than 20 mM. In addition, the decrease of neuronal activity at a high glucose concentration is not influenced by the blockade of inhibitory synaptic activity. These results suggest that a cultured neuronal network has optimal glucose concentration 17.56 mM, which corresponds to the concentration of glucose in a culture medium.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.133 No.10 （2013） 特集：分散エネルギーマネジメント

本誌掲載ページ: 1912-1917 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/133/10/133_1912/_article/-char/ja/