細胞運動の突出過程におけるアクチンダイナミクス解析

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2014/02/01

タイトル(英語): Analysis of Actin Protein Dynamics at the Protrusion Process of Cell Movement

著者名: 三浦　拓也（千葉大学），菅原　路子（千葉大学），八木　透（東京工業大学），坪田　健一（千葉大学），劉　浩（千葉大学）

著者名(英語): Takuya Miura (Chiba University), Michiko Sugawara (Chiba University), Tohru Yagi (Tokyo Institute of Technology), Ken-ichi Tsubota (Chiba University), Hao Liu (Chiba University)

キーワード: 細胞遊走，突出，アクチン，ストレスファイバ　　Cell movement，Protrusion，Actin，Stress fiber

要約(英語): Actin, a cytoskeletal protein, gathers and polymerizes under the cell membrane to mediate protrusion of the cell membrane. Therefore, actin participates greatly in cell movement. To quantitatively evaluate actin's dynamics during cell protrusion, movements of actin-labeled cells were observed under a confocal laser scanning microscopy, and time-lapse images were obtained. Image analysis of the orientation of actin stress fibers revealed dynamic formation of a meshwork of stress fibers in the protrusion structure at the leading edge of the motile cell. In addition, a number of immobile spots such as focal adhesions were observed adjacent to the dynamic meshwork of stress fibers. The distribution and the time between appearance and disappearance of each spot were analyzed. The results suggest that immobile spots close to the centroid of the cell play a crucial role in providing anchorage and driving cell movement.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.134 No.2 （2014） 特集：知覚情報技術の最前線

本誌掲載ページ: 177-182 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/134/2/134_177/_article/-char/ja/