三角くぼみ形状を利用した固定微小液滴への単細胞カプセル化に関する研究

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2014/06/01

タイトル(英語): A Study on Encapsulation of Single-Cell into Fixed Droplets Using Triangular Microwells

著者名: 服部　篤志（東京工業大学），八木　透（東京工業大学），曹　嘉文（國立中央大學）

著者名(英語): Atsushi Hattori (Yagi Labs., Tokyo Institute of Technology), Tohru Yagi (Yagi Labs., Tokyo Institute of Technology), Chia-Wen Tsao (µF2 Labs., National Central University)

キーワード: マイクロ流体デバイス，微小液滴，単細胞カプセル化　　Microfluidics，Droplet，Single cell encapsulation

要約(英語): Microfluidic devices using droplets are possible to perform high throughput screening of single-cell samples through encapsulating cells into droplets. In conventional encapsulation methods, devices have T-junction and flow-focusing geometry for droplet generation. Those techniques are difficult to achieve uniform distribution of cells, or to place a single-cell in droplets. Moreover, during screening samples, it is necessary to have a mechanism to hold droplets at one place for long time. It is, however, slightly hard to add such a function in conventional devices. Thus, we propose a new encapsulation method of single-cell using triangular microwells. In this approach, each single-cell is isolated, encapsulated, and held at one place using triangular microwells. This approach has benefits that the droplets size can be regulated by the size of microwells. In addition, the optimization of encapsulation is easier than conventional methods because the flow rate of oil phase does not affect to the encapsulation.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.134 No.6 （2014） 特集：デペンダブルなサービスシステムに貢献する情報・システム技術

本誌掲載ページ: 848-855 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/134/6/134_848/_article/-char/ja/