活動依存性マンガン造影MRIのための神経細胞内へのマンガン流入量の検討

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2015/03/01

タイトル(英語): Evaluation of the Amount of the Manganese Entry in the Neurons for Activity-induced Manganese-enhanced MRI

著者名: 菊田　里美（東北大学 大学院医学系研究科），柳川　右千夫（群馬大学 大学院医学系研究科），本間　経康（東北大学 大学院医学系研究科），小山内　実（東北大学 大学院医学系研究科）

著者名(英語): Satomi Kikuta (Graduate School of Medicine, Tohoku University), Yuchio Yanagawa (Graduate School of Medicine, Gunma University), Noriyasu Homma (Graduate School of Medicine, Tohoku University), Makoto Osanai (Graduate School of Medicine, Tohoku University)

キーワード: MRI，マンガン，カルシウムイメージング　　MRI，manganese，calcium imaging

要約(英語): The activity-induced manganese-enhanced MRI (AIM-MRI) is one of the candidate methods for measuring the neuronal activity noninvasively in vivo. This method has the potential to measure the history of the neuronal activity, since the Mn2+ can enter the active neuron through voltage-dependent Ca2+ channels. However, it has not been clear that the relation between the neuronal activity and the accumulation of Mn2+ in the cell. At first, we confirmed that the longitudinal relaxation time of H+ (T1) measured by means of MRI apparatus was related to the [Mn2+] using the pseudo intracellular phantoms with various concentration of Mn2+. The inverse of T1 (1/T1) was proportional to [Mn2+], thus [Mn2+] can be measured by MRI quantitatively. Next, we investigated the relation between the neuronal activity and the accumulation of Mn2+ in the striatal GABAergic neurons using the method of the Mn2+ quenching of Fura-2 LR, a family of fura fluorescent Ca2+ indicators. The amount of the Ca2+ influx was correlated to the neuronal activity evoked by tetanic stimulations with various frequencies. In the same slice preparation, the amounts of the fluorescence quench induced by the tetanic stimulations under the condition of 50μM MnCl2 administration were recorded. As a result, the amount of the quench was proportional to the amount of the Ca2+ influx. These results suggested that a cell, which has higher activity, accumulated larger amount of Mn2+ in the neuron. Thus, our results supported that AIM-MRI can measure the history of the neuronal activities in the whole brain noninvasively in vivo.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.135 No.3 （2015）

本誌掲載ページ: 280-284 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/135/3/135_280/_article/-char/ja/