培養海馬ニューロン新生の制御によるパターン分離能力の向上

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2015/07/01

タイトル(英語): Improvement in Pattern Separation by Regulating Neurogenesis in Hippocampal Culture

著者名: 田中　幸美（東京大学大学院 新領域創成科学研究科），門倉　智之助（東京大学大学院 新領域創成科学研究科），磯村　拓哉（東京大学大学院 新領域創成科学研究科），榛葉　健太（東京大学大学院 新領域創成科学研究科），小谷　潔（東京大学先端科学技術研究センター／独立行政法人科学技術振興機構, さきがけ），神保　泰彦（東京大学大学院 工学系研究科）

著者名(英語): Yukimi Tanaka (Graduate School of Frontier Sciences, The University of Tokyo), Tomonosuke Kadokura (Graduate School of Frontier Sciences, The University of Tokyo), Takuya Isomura (Graduate School of Frontier Sciences, The University of Tokyo), Kenta Shimba (Graduate School of Frontier Sciences, The University of Tokyo), Kiyoshi Kotani (Research Center for Advanced Science and Technology, The University of Tokyo/JST, PRESTO), Yasuhiko Jimbo (Graduate School of Engineering, The University of Tokyo)

キーワード: 成体ニューロン新生，パターン分離，微小電極アレイ，空間パターン刺激，活動依存シナプス可塑性　　adult neurogenesis，pattern separation，microelectrode array，spatial-pattern stimulation，activity-dependent synaptic plasticity

要約(英語): The newly generated neurons in adult animals are known to enhance the pattern separation capability. However, it remains unclear how the new-born neurons change the activity patterns in neuronal networks during the pattern separation. To verify the change of the whole neuronal network activity, the in vitro neurogenesis model is useful because of its observability and controllability. To develop the in vitro neurogenesis model, we modulated the amount of newly generated neurons in cultures by applying basic fibroblast growth factor (bFGF), or cytarabine (Ara-C). To investigate the pattern separation capability, the neural cultures were tetanized with two spatial-pattern stimuli, and then the change in neural response evoked by different pattern was evaluated using microelectrode array. We confirmed that the doublecortin expression in cultures with bFGF was up-regulated than that with Ara-C, indicating that bFGF promoted the neurogenesis. We found that the enhanced neurogenesis contributed the clusters of neuronal networks to specialize their responses to one of two spatial-pattern stimuli after tetanization, while the entire response tended to shift toward the immediately preceding pattern without the neurogenesis. These results suggest that the property of newly generated neurons that they only respond to the specific pattern unlike mature neurons enhances the pattern separation capability of the entire network.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.135 No.7 （2015） 特集：平成26 年電子・情報・システム部門大会

本誌掲載ページ: 805-812 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/135/7/135_805/_article/-char/ja/