微細加工を用いた副交感神経細胞-心筋細胞共培養系の構築

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2015/07/01

タイトル(英語): Co-culture of Parasympathetic Neurons and Cardiomyocyte

著者名: 大岩　孝輔（東京大学大学院 新領域創成科学研究科），榛葉　健太（東京大学大学院 新領域創成科学研究科），沼田　崇志（東京大学 先端科学技術研究センター），武内　彬正（東京大学大学院 新領域創成科学研究科），小谷　潔（東京大学 先端科学技術研究センター／科学技術振興機構 さきがけ），神保　泰彦（東京大学大学院 工学系研究科）

著者名(英語): Kosuke Oiwa (Graduate School of Frontier Sciences, The University of Tokyo), Kenta Shimba (Graduate School of Frontier Sciences, The University of Tokyo), Takashi Numata (Research Center for Advanced Science and Technology, The University of Tokyo), Akimasa Takeuchi (Graduate School of Frontier Sciences, The University of Tokyo), Kiyoshi Kotani (Research Center for Advanced Science and Technology, The University of Tokyo/JST, PRESTO), Yasuhiko Jimbo (Graduate School of Engineering, The University of Tokyo)

キーワード: 心臓神経節，心房筋細胞，微小電極アレイ，共培養系，電気刺激，薬理遮断　　Intracardiac ganglion，Atrial myocytes，Microelectrode-array，Co-culture system，Electrical stimulation，Pharmacological blockade

要約(英語): The sympathetic neurons (SN) and the parasympathetic neurons (PN) have important roles in regulation of the cardiac activity. Imbalance of the SN and the PN activities results in abnormal cardiac electrophysiology and can cause several diseases such as atrial fibrillation. While activities of SN and cardiomyocytes have been studied using microfabrication technique, relationship between activities of PN and cardiomyocytes remains unclear. In this study, we developed a device for co-culture of intracardiac ganglion (ICG) neurons which are parasympathetic neurons and atrial myocytes (AMs) using microfabrication technique. Morphological connections between ICG neurons and AMs were observed by immunostaining. Inter-beat-intervals (IBI) were modulated after electrical stimulation of ICG neurons. This modulation completely blocked after applying atropine, a muscarinic receptor antagonist. These results suggest that the co-culture system can be utilized in the research of cell-cell interactions between parasympathetic neurons and cardiomyocytes.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.135 No.7 （2015） 特集：平成26 年電子・情報・システム部門大会

本誌掲載ページ: 813-818 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/135/7/135_813/_article/-char/ja/