2次元パターニングによるヒトiPSC由来神経細胞の移動制御

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2016/09/01

タイトル(英語): Migration Guidance of Human iPSC-derived Neurons by a Two-dimensional Patterning

著者名: 飯田　尚子（東京大学大学院 工学系研究科），榛葉　健太（東京大学大学院 新領域創成科学研究科），田中　幸美（東京大学大学院 新領域創成科学研究科），酒井　洸児（東京大学大学院 工学系研究科），小谷　潔（東京大学 先端科学技術研究センター／JST さきがけ），神保　泰彦（東京大学大学院 工学系研究科）

著者名(英語): Shoko Iida (School of Engineering, The University of Tokyo), Kenta Shimba (Graduate School of Frontier Sciences, The University of Tokyo), Yukimi Tanaka (Graduate School of Frontier Sciences, The University of Tokyo), Koji Sakai (School of Engineering, The University of Tokyo), Kiyoshi Kotani (Research Center for Advanced Science and Technology, The University of Tokyo/JST PRESTO), Yasuhiko Jimbo (School of Engineering, The University of Tokyo)

キーワード: 大脳皮質，層形成，細胞移動，人工多能性幹細胞，マイクロパターニング，ラミニン　　Cerebral cortex，cortical layer formation，cell migration，induced pluripotent stem cell，micro-patterning，laminin

要約(英語): The layered structure in the cerebral cortex has an important role in expressing brain function and is formed by cell migration during embryogenesis. However, it is not clear how neurons migrate and form layers orderly because an appropriate culture method is yet to be developed for evaluating cell migration. Our purpose is to develop an experimental system to control directions of human-neuronal migration in two-dimensional culture in order to understand the mechanism of neuronal migration during corticogenesis. Here, we made a stripe pattern of laminin on culture dishes, and cultured human induced pluripotent stem cell (iPSC)-derived neural cells on the pattern. iPSC-derived neural stem cells and neurons extended their fibers along the pattern. Then, migration of neural cells on the pattern was traced with time-lapse imaging. As a result, the migration length along the pattern was significantly longer than that orthogonal to, suggesting that the laminin pattern guides effectively neural-cell migration. Our method using the laminin pattern should be appropriate for controlling and observing human-neural-cell migration, and moreover, applicable to the study of revealing the mechanism of the migration.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.136 No.9 （2016） 特集：神経工学・サイバネティックス・生体工学

本誌掲載ページ: 1268-1276 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/136/9/136_1268/_article/-char/ja/