3次元Lab-on-a-CD免疫センシングにおける高感度化

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2017/03/01

タイトル(英語): A study for Sensitivity Improvement of 3-D Lab-on-a-CD based Immunosensor

著者名: 片岡　千和（（同）カーバンクル・バイオサイエンテック／兵庫県立大学 高度産業科学技術研究所），畔田　吏（兵庫県立大学 高度産業科学技術研究所），澤田石　一之（（同）カーバンクル・バイオサイエンテック），浮田 　芳昭（山梨大学工学部機械工学科），山口　明啓（兵庫県立大学 高度産業科学技術研究所），内海　裕一（兵庫県立大学 高度産業科学技術研究所）

著者名(英語): Chiwa Kataoka (Carbuncle BioScienTech LLC./Laboratory of Advanced Science and Technology for Industry (LASTI), University of Hyogo), Tsukasa Azeta (Laboratory of Advanced Science and Technology for Industry (LASTI), University of Hyogo), Kazuyuki Sawadaishi (Carbuncle BioScienTech LLC.), Yoshiaki Ukita (Department of Interdisciplinary Research, Graduate School of University of Yamanashi), Akinobu Yamaguchi (Laboratory of Advanced Science and Technology for Industry (LASTI), University of Hyogo), Yuichi Utsumi (Laboratory of Advanced Science and Technology for Industry (LASTI), University of Hyogo)

キーワード: LIGAプロセス，3次元Lab-on-a-CD，免疫測定，高感度化　　LIGA process，3D Lab-on-a-CD，immunoassay，sensitivity improvement

要約(英語): We have developed a 3D Lab-on-a-CD immunosensor based on a competitive/non-competitive assay for small endocrine disruptors and biomolecules. To improve the sensitivity of the 3D Lab-on-a-CD immunosensor, an immobilizing method of capturing-type antibody on the sensor surface was investigated. The method is based on the introduction of a highly reactive amino group to a P(MMA/CEMA) copolymer surface having chlorine, and utilizing a biotin-streptavidin system. The functionality of the 3D Lab-on-a-CD immunosensor was evaluated by measuring mouse IgG. The detection limit was found to be 3 f g/assay (3×10^-12 g/assay). After further modification, the 3D Lab-on-a-CD immunosensor, having some characteristics such as a substrate with highly integrated antibodies, is expected to be applicable for highly sensitive immunoassay.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.137 No.3 （2017） 特集：量子ビームによるナノバイオサイエンス技術

本誌掲載ページ: 418-423 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/137/3/137_418/_article/-char/ja/