高密度CMOSアレイ上の分散培養系における活動電位波形に基づく興奮性及び抑制性神経細胞の分類

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2019/05/01

タイトル(英語): Classification of Inhibitory and Excitatory Neurons of Dissociated Cultures Based on Action Potential Waveforms on High-density CMOS Microelectrode Arrays

著者名: 三田　毅（東京大学大学院情報理工学系研究科），Douglas　Bakkum（チューリッヒ工科大学Biosystems Science and Engineering学科），Urs　Frey（理化学研究所生命システム研究センター），Andreas　Hierlemann（チューリッヒ工科大学Biosystems Science and Engineering学科），神崎　亮平（東京大学大学院情報理工学系研究科／東京大学先端科学技術研究センター），高橋　宏知（東京大学大学院情報理工学系研究科／東京大学先端科学技

著者名(英語): Takeshi Mita (Graduate School of Information Science and Technology, The University of Tokyo), Douglas Bakkum (Department of Biosystems Science and Engineering, ETH Zurich), Urs Frey (RIKEN Quantitative Biology Center), Andreas Hierlemann (Department of Biosystems Science and Engineering, ETH Zurich), Ryohei Kanzaki (Graduate School of Information Science and Technology, The University of Tokyo/Research Center for Advanced Science and Technology, The University of Tokyo), Hirokazu Takahashi (Graduate School of Information Science and Technology, The University of Tokyo/Research Center for Advanced Science and Technology, The University of Tokyo)

キーワード: 分散培養，抑制性神経細胞，CMOS，微小電極アレイ，細胞外計測　　dissociated culture，inhibitory neuron，CMOS，microelectrode array，extracellular recording

要約(英語): Electrophysiological data from in vivo and slice preparations show that inhibitory neurons had shorter duration action potentials (AP) than excitatory neurons. However, this criterion has not yet been established in dissociated cultured neurons. In the present study, we used a high-density CMOS microelectrode array to extracellularly investigate neural signals in primary dissociated cultures of rat neocortex, and we characterized AP waveforms to discriminate excitatory and inhibitory neurons. The CMOS array offers the possibility to acquire comprehensive spatio-temporal neural activity patterns with 11,011 electrodes in about 2×1.75 mm2 area at 20-kHz sampling rate. The waveforms of APs were investigated around cell bodies of neurons, which were classified into either excitatory neurons or inhibitory neurons on the basis of MAP2 and GABA immunostaining images. Consistent with previous in vivo and slice studies, we demonstrated that AP waveforms of inhibitory neurons had shorter durations and recovery time than those of excitatory neurons. The discrimination accuracy was around 0.9 in the receiver-operating characteristics (ROC) analyses. Additionally, taking advantage of non-invasive CMOS recording, we investigated AP waveforms throughout development of cultures. We confirmed that APs were classified into two classes, i.e., putative excitatory and inhibitory neurons, regardless of developmental stages, and found that the duration and recovery time of AP shortened in matured cultures. Thus, AP waveforms have rich information about cell types and developmental stages, which are of worth to elucidate underlying mechanisms of neuronal dynamics in spatio-temporal patterns.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.139 No.5 （2019） 特集Ⅰ：電子・情報・システム技術とものづくり　特集Ⅱ：生体を診る・解く・支える技術

本誌掲載ページ: 615-624 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/139/5/139_615/_article/-char/ja/