ナノポア支持型脂質二重膜の自発展開法

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2019/10/01

タイトル(英語): Nano-pore Lipid Bilayer Formed by Self-spreading Method

著者名: 彭　祖癸（東京工業大学 工学院・機械系），榛葉　健太（東京大学大学院 工学系研究科・精密工学専攻），宮本　義孝（東京工業大学 工学院・機械系），八木　透（東京工業大学 工学院・機械系）

著者名(英語): Zugui Peng (School of Engineering Department of Mechanical Engineering, Tokyo Institute of Technology), Kenta Shimba (School of Engineering, The University of Tokyo), Yoshitaka Miyamoto (School of Engineering Department of Mechanical Engineering, Tokyo Institute of Technology), Tohru Yagi (School of Engineering Department of Mechanical Engineering, Tokyo Institute of Technology)

キーワード: 脂質二重膜，自発展開法，多孔質フィルム，原子間力顕微鏡，膜タンパク質　　lipid bilayer，self-spreading method，membrane filter，atomic force microscope，membrane protein

要約(英語): Black lipid membranes suspending on a solid-state nanopore (nano black lipid membranes; nano-BLMs) is a functional application to detect single-channel activities with a long lifetime. However, folding and painting techniques, which are traditionally used to form nano-BLMs, only have a low success rate since its complicated procedure. Herein, we report on the self-spreading method on 50 nm pore size polycarbonate membrane filter to form nano-BLMs by a simple procedure. Our results show that the nano-BLMs have been successfully formed on the membrane filter. The spreading velocity of the nano-BLMs exhibits a square root behavior, v～t-1/2, which is in a great agreement with the previous research. The experiments with fluorescence recovery after photobleaching (FRAP) show the recovery of a photobleached area in our nano-BLMs. We also measure the elastic response of our nano-BLMs by using an atomic force microscope (AFM), and our results demonstrate that the lipid bilayer is spanning on the pore of membrane filter. We also discuss the effect of temperature and the roughness of supporting surface on the spreading speed of lipid bilayer.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.139 No.10 （2019） 特集Ⅰ：インテリジェント・システム　特集Ⅱ：2018電気関係学会四国支部連合大会

本誌掲載ページ: 1146-1152 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/139/10/139_1146/_article/-char/ja/