高電界パルス印加による大腸菌および酵母内の核酸抽出
高電界パルス印加による大腸菌および酵母内の核酸抽出
カテゴリ: 論文誌(論文単位)
グループ名: 【C】電子・情報・システム部門
発行日: 2020/12/01
タイトル(英語): Extraction of Nucleic Acid in Escherichia coli and Saccharomyces cerevisiae Using High Electric Field Pulse Application
著者名: 村上 祐一(名城大学 理工学部 電気電子工学科),山下 遼(名城大学 理工学部 電気電子工学科),佐藤 拓直(名城大学 理工学部 電気電子工学科),村本 裕二(名城大学 理工学部 電気電子工学科)
著者名(英語): Yuichi Murakami (Department of Electrical and Electronic Engineering, Faculty of Science and Technology, Meijo University), Ryo Yamashita (Department of Electrical and Electronic Engineering, Faculty of Science and Technology, Meijo University), Takunao Sato (Department of Electrical and Electronic Engineering, Faculty of Science and Technology, Meijo University), Yuji Muramoto (Department of Electrical and Electronic Engineering, Faculty of Science and Technology, Meijo University)
キーワード: 抽出方法,高電界パルス,核酸 extraction method,high electric field pulse,nucleic acid
要約(英語): An electroporation that formed the pores on cell membrane after application of high electric field pulses has been used the transformation and the sterilization of cell. In this paper, we reported the extraction method of nucleic acid in Escherichia coli and Saccharomyces cerevisiae using high electric field pulse application. The sample was 1.0×10-3 mol/L NaCl aqueous solution contained E. coli and S. cerevisiae. High voltage pulse is applied to the sample at room temperature. These results were shown as the following: (1) When a pulsed electric field of 4~13 kV/mm is applied to E. coli and S. cerevisiae, the estimated amount of nucleic acid increases with the increases of strength of applied pulsed electric field. (2) In the case of E. coli, the 2-3 kbp band was confirmed at an electric field of 5 kV/mm or more, and the 10 kbp band was confirmed at an electric field of 8 kV/mm or more. (3) In the case of S. cerevisiae, the 2-3 kbp band was confirmed at an electric field of 9 kV/mm or more. the 10 kbp band could not be confirmed at 13 kV/mm or less.
本誌: 電気学会論文誌C(電子・情報・システム部門誌) Vol.140 No.12 (2020) 特集:電気・電子・情報関係学会東海支部連合大会
本誌掲載ページ: 1336-1342 p
原稿種別: 論文/日本語
電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/140/12/140_1336/_article/-char/ja/
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