Formation of Agarose-supported Liposomes by Polymer-assisted Method toward Biosensor

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2021/05/01

タイトル(英語): Formation of Agarose-supported Liposomes by Polymer-assisted Method toward Biosensor

著者名: Zugui Peng (School of Engineering, Tokyo Institute of Technology), Kentaro Wada (School of Engineering, Tokyo Institute of Technology), Kenta Shimba (School of Engineering, The University of Tokyo), Yoshitaka Miyamoto (Department of Reproductive Biology,

著者名(英語): Zugui Peng (School of Engineering, Tokyo Institute of Technology), Kentaro Wada (School of Engineering, Tokyo Institute of Technology), Kenta Shimba (School of Engineering, The University of Tokyo), Yoshitaka Miyamoto (Department of Reproductive Biology,

キーワード: liposome，agarose，cytoskeletal-mimic material，polymer-assisted hydration，biosensor

要約(英語): Liposomes are spherical structures made from two membranes of phospholipid molecules enclosing an aqueous compartment. Since their components and structures are close to those of cell membranes, one can modify liposomes with membrane protein. This makes liposomes ideal candidate as biosensors. However, due to the absence of cytoskeleton, liposomes are very fragile. Its poor mechanical property greatly restricts their use. For this reason, encapsulation of cytoskeletal-mimic materials into liposomes is highly desirable. Here, we develop a method to encapsulate agarose as a cytoskeletal-mimic material into liposomes by using a polymer-assisted hydration approach. We show that by our method, a large number of agarose-supported liposomes can be formed without any complicated procedures. Experimental results indicate that the performed liposomes have a higher mechanical stability than non-supported liposomes. At the same time, we show that transmembrane proteins are still able to bind to the lipid membrane, and small molecules can diffuse through the membrane into the liposome interior.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.141 No.5 （2021） 特集：神経工学

本誌掲載ページ: 646-653 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/141/5/141_646/_article/-char/ja/