電気活動計測を用いた培養感覚神経細胞に対する感作性評価手法の開発

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2023/07/01

タイトル(英語): Developing Evaluation Method for Peripheral Sensitization in Cultured Sensory Neurons

著者名: 宮原　優希（東京大学大学院 工学系研究科），榛葉　健太（東京大学大学院 工学系研究科），小谷　潔（東京大学 先端科学技術研究センター），神保　泰彦（東京大学大学院 工学系研究科）

著者名(英語): Yuki Miyahara (School of Engineering, The University of Tokyo), Kenta Shimba (School of Engineering, The University of Tokyo), Kiyoshi Kotani (Research Center for Advanced Science and Technology, The University of Tokyo), Yasuhiko Jimbo (School of Engineering, The University of Tokyo)

キーワード: 痛み，末梢性感作，高密度微小電極アレイ，感覚神経細胞，サブスタンスP　　pain，peripheral sensitization，high-density microelectrode array，sensory neuron，substance P

要約(英語): Peripheral sensitization, decrease in pain threshold in sensory neurons, can cause chronic pain. Little is known about how peripheral sensitization led to chronic pain. Here, we aimed to develop a method for evaluating peripheral sensitization in cultured sensory neurons with electrical recording. Sensory neurons from rat dorsal root ganglion were cultured on high-density microelectrode arrays, and their activity was evoked by capsaicin stimulation with or without substance P. Fluorescent imaging-based electrode selection effectively selected five times as many capsaicin-sensitive sensory neurons as the existing method. Peripheral sensitization by substance P was detected from 31.9% of selected sensory neurons, and majority of these neurons co-expressed capsaicin and substance P receptors. These results indicate that our method is suitable for evaluating peripheral sensitization by substance P in cultured sensory neurons.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.143 No.7 （2023） 特集：2022年電子・情報・システム部門大会

本誌掲載ページ: 641-648 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/143/7/143_641/_article/-char/ja/