Rapid Diagnostic Device for Subclinical Mastitis Based on Electrochemical Detection of Superoxide Produced from Neutrophils in Fresh Milk

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2011/06/01

タイトル(英語): Rapid Diagnostic Device for Subclinical Mastitis Based on Electrochemical Detection of Superoxide Produced from Neutrophils in Fresh Milk

著者名: Kohei Okada (Graduate School of Pure and Applied Sciences, University of Tsukuba), Junji Fukuda (Graduate School of Pure and Applied Sciences, University of Tsukuba), Hiroaki Suzuki (Graduate School of Pure and Applied Sciences, University of Tsukuba)

著者名(英語): Kohei Okada (Graduate School of Pure and Applied Sciences, University of Tsukuba), Junji Fukuda (Graduate School of Pure and Applied Sciences, University of Tsukuba), Hiroaki Suzuki (Graduate School of Pure and Applied Sciences, University of Tsukuba)

キーワード: Mastitis，Superoxide，Self-assembled monolayer，Superoxide dismutase

要約(英語): Electrochemical microdevices were fabricated to identify mastitic cows based on the increased number of neutrophils in raw milk. Because neutrophils produce superoxide (O2・-(, the amount of O2・- can be used as an early indicator for subclinical mastitis. In the microdevices, O2・- was detected on a gold electrode using superoxide dismutase immobilized via a self-assembled monolayer of cysteine. In a preliminary test using xanthine oxidase to produce O2・-, one of the devices detected the production and rapid extinction of O2・-. When neutrophils obtained from a mastitic cow were concentrated by centrifugation and introduced into the device, a current increase distinctly different from the background was observed. Furthermore, a micropillar structure was fabricated on the gold electrode to trap and collect neutrophils, thereby facilitating the concentration of these cells around the electrode. The measured current clearly depended on the number of neutrophils in raw milk samples, demonstrating the applicability of the device for rapid diagnosis of subclinical mastitis.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.131 No.6 （2011） 特集：農業センサシステム

本誌掲載ページ: 218-222 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/131/6/131_6_218/_article/-char/ja/