接触法による膜たんぱく質のアレイ化

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2011/12/01

タイトル(英語): Simple and Stable Lipid Bilayer Formation: A Droplets Contacting Method using Parylene Micro-pores for Multiple Ion Channel Recordings

著者名: 辻 祐太郎（神奈川科学技術アカデミー／慶應義塾大学理工学部），川野 竜司（神奈川科学技術アカデミー），大崎 寿久（神奈川科学技術アカデミー），佐々木啓孝（神奈川科学技術アカデミー），三木 則尚（神奈川科学技術アカデミー／慶應義塾大学理工学部），竹内 昌治（神奈川科学技術アカデミー／東京大学生産技術研究所）

著者名(英語): Yutaro Tsuji (Kanagawa Academy of Science and Technology/Keio University), Ryuji Kawano (Kanagawa Academy of Science and Technology), Toshihisa Osaki (Kanagawa Academy of Science and Technology), Hirotaka Sasaki (Kanagawa Academy of Science and Technology), Norihisa Miki (Kanagawa Academy of Science and Technology/Keio University), and Shoji Takeuchi (Kanagawa Academy of Science and Technology/Institute of Industrial Science, The University of Tokyo)

キーワード: 脂質二重膜，膜たんぱく質，接触法，創薬スクリーニング，μTAS　　Bilayer lipid membranes，Membrane protein，Droplets Contacting Method，Drug screening，Micro total analysis system

要約(英語): This paper describes multiple ion-channel recordings through membrane proteins reconstituted in bilayer lipid membranes (BLMs) array. The BLMs array can be prepared by “Droplets Contacting Method” which forms BLMs at the interface of two lipid monolayers. Since this method does not require skilled techniques, it is highly reproducible and can be applied to automated system. We used a double well chip (DWC) for the droplets contacting method. We attempted to confine the BLMs forming areas with parylene micro-pore (parylene double well chip, PDWC) to augment the mechanical stability of BLMs. Subsequently, we arrayed the PDWC with electrodes for multiple recordings of channel proteins. We successfully demonstrated 14 channels simultaneous ion channel recordings through α-hemolysin.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.131 No.12 （2011） 特集：最先端半導体技術応用バイオメディカルデバイス

本誌掲載ページ: 419-424 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/131/12/131_12_419/_article/-char/ja/