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共焦点光学系を用いたマイクロウェル内微量蛍光体の検出感度向上

共焦点光学系を用いたマイクロウェル内微量蛍光体の検出感度向上

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カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2013/06/01

タイトル(英語): Improvement of Detection Sensitivity for Small Amount Fluorescence Dyes in Microwell by Confocal Optics System

著者名: 安藤 毅(埼玉大学大学院理工学研究科),木下 保則(埼玉大学大学院理工学研究科),Ahmed Shamim(埼玉大学大学院理工学研究科),西垣 功一(埼玉大学大学院理工学研究科),内田 秀和(埼玉大学大学院理工学研究科)

著者名(英語): Ki Ando (The Graduate School of Science and Engineering, Saitama University), Yasunori Kinoshita (The Graduate School of Science and Engineering, Saitama University), Ahmed Shamim (The Graduate School of Science and Engineering, Saitama University), Koichi Nishigaki (The Graduate School of Science and Engineering, Saitama University), Hidekazu Uchida (The Graduate School of Science and Engineering, Saitama University)

キーワード: 共焦点,マイクロプレート,マイクロアレイ,蛍光寿命測定,時間分解蛍光測定  confocal,microplate,microarray,fluorescence lifetime imaging microscopy (FLIM),time-resolved fluorescence (TRF)

要約(英語): In this study, we worked on the improvement of detection sensitivity for fluorescence dyes filled in the well of Microwell with Manageable Volume (MMV). For this purpose, we applied fluorescence lifetime imaging microscopy (FLIM) and time-resolved fluorescence (TRF) to method of fluorescence detection. In addition, we built up confocal optics system with a pinhole in front of the photomultiplier tube on the fluorescence microscopy in order to decrease noise from MMV. In the result, the confocal optics system increased the S/N ratio of photon count from dilute Alexa fluor 430. However, the pinhole limited available photon and mechanical vignetting of light path by lateral face of the well also restricted available photon. They caused increasing standard deviation of the result. These issues affected sensitivity of fluorescence detection and it is necessary to balance between noise reduction and amount of available photon for improvement of detection sensitivity by confocal optics system. We suggested suitable pinhole size and other parameters in order to keep the balance, and we succeeded to detect 1 pM Alexa fluor 430 filled into the well of MMV.

本誌: 電気学会論文誌E(センサ・マイクロマシン部門誌) Vol.133 No.6 (2013) 特集:匂い・香りのセンシングおよび再現技術の新展開

本誌掲載ページ: 250-257 p

原稿種別: 論文/日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/133/6/133_250/_article/-char/ja/

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