Effect of Microfluidic Channel on Sensitivity and Response in Gold-linked Electrochemical Immunoassay

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2013/12/02

タイトル(英語): Effect of Microfluidic Channel on Sensitivity and Response in Gold-linked Electrochemical Immunoassay

著者名: Suguru Shiohara (Japan Advanced Institute of Science and Technology), Miyuki Chikae (Japan Advanced Institute of Science and Technology), Yoshiaki Ukita (Japan Advanced Institute of Science and Technology), Hiromi Ushijima (Biodevice Technology Ltd.), Eii

著者名(英語): Suguru Shiohara (Japan Advanced Institute of Science and Technology), Miyuki Chikae (Japan Advanced Institute of Science and Technology), Yoshiaki Ukita (Japan Advanced Institute of Science and Technology), Hiromi Ushijima (Biodevice Technology Ltd.), Eiichi Tamiya (Osaka University), Yuzuru Takamura (Japan Advanced Institute of Science and Technology)

キーワード: Microchannel，Gold-nano-particle，POCT，Immunoassay

要約(英語): With the aim of developing compact and sensitive immunoassay devices for point-of-care testing, we studied an electrochemical method by using continuous flow in a microchannel gold-linked electrochemical immunoassay (GLEIA) on a screen-printed carbon electrode. Conventional GLEIA is a useful method to detect low-level antigen in real samples, but it requires a long incubation time (～1h) and milliliter-order reagent volumes for measurement. In this study, a screen-printed electrode was put into a microfluidic channel to study the microchannel effect on GLEIA. By limiting the diffusion volume during the pre-oxidation to oxidation step of GLEIA, we found that the sensitivity increased. Owing to the continuous flow in a microchannel, the antigen-antibody reaction was accelerated, resulting in a 10 min detection time. It was also effective in reducing the minimum volume of washing buffer required.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.133 No.12 （2013） 特集：マイクロ・ナノ流体デバイス開発とその応用

本誌掲載ページ: 344-349 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/133/12/133_344/_article/-char/ja/