高精度細胞伸展マイクロデバイスの開発と引張りを受ける細胞のその場観察
高精度細胞伸展マイクロデバイスの開発と引張りを受ける細胞のその場観察
カテゴリ: 論文誌(論文単位)
グループ名: 【E】センサ・マイクロマシン部門
発行日: 2013/12/02
タイトル(英語): Development of Highly Precise Cell Stretching Microdevice and in-situ Observation of Stretched Cell
著者名: 中島 雄太(熊本大学 大学院自然科学研究科/山口大学 大学院理工学研究科),門司 亮(山口大学 大学院理工学研究科),佐藤 克也(徳島大学 大学院ソシオテクノサイエンス研究部),南 和幸(山口大学 大学院理工学研究科)
著者名(英語): Yuta Nakashima (Graduate School of Science and Technology, Kumamoto University/Graduate School of Science and Engineering, Yamaguchi University), Ryo Monji (Graduate School of Science and Engineering, Yamaguchi University), Katsuya Sato (Institute of Technology and Science, The University of Tokushima), Kazuyuki Minami (Graduate School of Science and Engineering, Yamaguchi University)
キーワード: 細胞伸展,力学刺激,細胞応答評価,細胞バイオメカニクス,マイクロデバイス Cell stretching,Mechanical stimulation,Cellular response evaluation,Cell biomechanics,Microdevice
要約(英語): This paper presents a cell stretching microdevice for in-situ observation of stretched cell. In a conventional cell stretching device, it was difficult to observe the initial and in-situ cellular response to stretching. The presented device consists of an elastic culture chamber and leaf springs by which uniaxial stretch is applied to the culture chamber. The device is produced by a micro three-dimensional structure fabrication process using multiple exposures to the photoresist. The device permits the observation of cellular responses to stretching in real time because it is made of transparent materials when observed by optical microscope. We demonstrated the fundamental characteristics of a microdevice, and observed the cellular response behavior by a fabricated microdevice. The amount of the culture chamber deformation was controlled by the amount of the stretching arm displacement. Also, we demonstrated the culture chamber during stretching was not deformed toward the optical axis. Moreover, in-situ observation of transition of the cellular calcium signaling response to stretching succeeded by optical microscopy using fabricated microdevice. These results indicated that this device can be used to observe and evaluate the initial cellular response and micro-strain field on a cell membrane during uniaxial stretching.
本誌: 電気学会論文誌E(センサ・マイクロマシン部門誌) Vol.133 No.12 (2013) 特集:マイクロ・ナノ流体デバイス開発とその応用
本誌掲載ページ: 350-357 p
原稿種別: 論文/日本語
電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/133/12/133_350/_article/-char/ja/
受取状況を読み込めませんでした
