商品情報にスキップ
1 1

細胞圧縮刺激応答評価マイクロデバイスを用いた細胞応答のリアルタイム観察と評価

細胞圧縮刺激応答評価マイクロデバイスを用いた細胞応答のリアルタイム観察と評価

通常価格 ¥770 JPY
通常価格 セール価格 ¥770 JPY
セール 売り切れ
税込

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2013/12/02

タイトル(英語): Observation and Evaluation of Real-time Cellular Responses Using a Cell Compression Stimulus Microdevice

著者名: 中島 雄太(熊本大学 大学院自然科学研究科/山口大学 大学院理工学研究科),楊 寅(山口大学 大学院理工学研究科),南 和幸(山口大学 大学院理工学研究科)

著者名(英語): Yuta Nakashima (Graduate School of Science and Technology, Kumamoto University/Graduate School of Science and Engineering, Yamaguchi University), Yin Yang (Graduate School of Science and Engineering, Yamaguchi University), Kazuyuki Minami (Graduate School of Science and Engineering, Yamaguchi University)

キーワード: 細胞圧縮刺激,細胞応答評価,リアルタイム観察,細胞バイオメカニクス,マイクロデバイス  Cell compression stimuli,Cellular response evaluation,Real-time observation,Cell biomechanics,Microdevice

要約(英語): This paper presents a cell compression stimulus microdevice intended to evaluate the real-time response of cells to compression stimuli. The presented device consists of cell inlet ports, a pressure inlet port, a gasket, microchannels, culture chambers, and a diaphragm on the culture chamber for applying compressive pressure to cells. Compression stimuli applied to the cells can be controlled by regulating the expansion of the diaphragm via a pressure control. The device permits the observation of cellular responses to compressive pressure in real time because it is made of transparent materials and stimulates the cells without deforming the cell culture surface, when observed by optical microscope. We observed the cellular deformation and response behavior using a fabricated microdevice in real time. The changes of intracellular calcium concentration before and after compressive stimuli were observed by the fabricated device. Also, the intracellular maximum strain part and strain distribution of a cell that received compression stimulus were clarified by FEM analysis. These results indicate the device is expected to clarify the cellular mechanoreceptor mechanisms and signal transduction pathways and facilitate the control of stem cell differentiation by a combination of the experimental results and FEM analysis results.

本誌: 電気学会論文誌E(センサ・マイクロマシン部門誌) Vol.133 No.12 (2013) 特集:マイクロ・ナノ流体デバイス開発とその応用

本誌掲載ページ: 358-364 p

原稿種別: 論文/日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/133/12/133_358/_article/-char/ja/

販売タイプ
書籍サイズ
ページ数
詳細を表示する