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Velocity Control of Microtubules with High Spatial Resolution on an Au-coated Surface with an SU-8 Thermal Isolation Layer

Velocity Control of Microtubules with High Spatial Resolution on an Au-coated Surface with an SU-8 Thermal Isolation Layer

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カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2016/03/01

タイトル(英語): Velocity Control of Microtubules with High Spatial Resolution on an Au-coated Surface with an SU-8 Thermal Isolation Layer

著者名: Tasuku Nakahara (Graduate School of Science and Engineering, Yamaguchi University/Department of Micro Engineering, Kyoto University), Hirofumi Shintaku (Department of Micro Engineering, Kyoto University), Hidetoshi Kotera (Department of Micro Engineering,

著者名(英語): Tasuku Nakahara (Graduate School of Science and Engineering, Yamaguchi University/Department of Micro Engineering, Kyoto University), Hirofumi Shintaku (Department of Micro Engineering, Kyoto University), Hidetoshi Kotera (Department of Micro Engineering, Kyoto University), Ryuji Yokokawa (Department of Micro Engineering, Kyoto University)

キーワード: Motor proteins,Molecular transport,Kinesin,Microtubule

要約(英語): The velocity of microtubules gliding on a kinesin-coated surface has been modulated by changing the rate of ATP hydrolysis, temperature, and buffer conditions. Conventional methods have not realized velocity control with high spatial resolution. Here, we propose a method to selectively determine the gliding velocity of microtubules with the help of light irradiance on an Au-coated Si surface. A part of the Au layer was thermally isolated by an underneath coating of SU-8 photoresist, which was employed as a thermal insulation layer. In the area without SU-8, the Au layer was deposited directly on the Si substrate. Irradiation of 13.5 W/cm2 controlled the velocity with approximately 40 μm of spatial resolution in the SU-8 coated area, resulting in a velocity of 0.80 ± 0.10 μm/s, whereas in the non-coated area the velocity was 0.62 ± 0.05 μm/s.

本誌: 電気学会論文誌E(センサ・マイクロマシン部門誌) Vol.136 No.3 (2016) 特集:センサ・マイクロマシン英文特集号

本誌掲載ページ: 77-82 p

原稿種別: 論文/英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/136/3/136_77/_article/-char/ja/

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